Cloned (Comment) | Organism |
---|---|
gene sod, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparison, phylogenetic tree, inducible expression in Pichia pastoris strain GS115 using the AOX1 promoter | Thermoascus aurantiacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by KCN and H2O2 | Thermoascus aurantiacus | |
NaN3 | - |
Thermoascus aurantiacus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the enzyme possesses a unique 35 amino acid mitochondrial targeting sequence at the N-terminus | Thermoascus aurantiacus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn | a manganese superoxide dismutase, contains 0.00246 mg Mn/mg protein, binding involves conserved residues H88, H136, D222, and H226 | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21700 | - |
4 * 21700, SDS-PAGE | Thermoascus aurantiacus |
68000 | - |
gel filtration | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | A3RLW7 | var. levisporus, gene sod | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain GS115 by anion exchange chromatography | Thermoascus aurantiacus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Thermoascus aurantiacus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2324 | - |
purified recombinant enzyme | Thermoascus aurantiacus |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 21700, SDS-PAGE | Thermoascus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
manganese superoxide dismutase | - |
Thermoascus aurantiacus |
MnSOD | - |
Thermoascus aurantiacus |
SOD | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Thermoascus aurantiacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | - |
Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | purified recombinant enzyme, stable | Thermoascus aurantiacus |
70 | - |
purified recombinant enzyme, 65% remaining activity after 60 min | Thermoascus aurantiacus |
80 | - |
purified recombinant enzyme, half-life is about 40 min | Thermoascus aurantiacus |
90 | - |
purified recombinant enzyme, 20% remaining activity after 50 min | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant enzyme | Thermoascus aurantiacus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | activity range | Thermoascus aurantiacus |