Literature summary for 1.15.1.1 extracted from

Nedeva, T.; Dolashka-Angelova, P.; Moshtanska, V.; Voelter, W.; Petrova, V.; Kujumdzieva, A.
Purification and partial characterization of Cu/Zn superoxide dismutase from Kluyveromyces marxianus yeast (2009), J. Chromatogr. B, 877, 3529-3536.

Search for more literature for 1.15.1.1

Inhibitors

Inhibitors	Comment	Organism	Structure
4-chloromercuribenzoate	26.6% inhibition at 1 mM	Kluyveromyces marxianus
EDTA	66% inhibition at 1 mM	Kluyveromyces marxianus
iodoacetic acid	23.5% inhibition at 1 mM	Kluyveromyces marxianus
KCN	30% inhibition at 2 mM	Kluyveromyces marxianus
additional information	no inhibition of Cu/ZnSOD by 10 mM H2O2, 1 mM CaCl2, and 1 mM NaN3	Kluyveromyces marxianus
NaCl	slight inhibition at 1 mM	Kluyveromyces marxianus
SDS	-	Kluyveromyces marxianus
ZnCl2	slight inhibition at 1 mM	Kluyveromyces marxianus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu	a Cu/Zn-SOD	Kluyveromyces marxianus
Zn	a Cu/Zn-SOD	Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34034	-	2 * 34034, sequence calculation and mass spectrometry	Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 O2.- + 2 H+	Kluyveromyces marxianus	-	O2 + H2O2	-	?
2 O2.- + 2 H+	Kluyveromyces marxianus NBIMCC 1984	-	O2 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Kluyveromyces marxianus	-	-	-
Kluyveromyces marxianus NBIMCC 1984	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme contains carbohydrate at 1200 kDa, it is associated with carbohydrate components comprising at least 4 monosugars, D-xylose, D-mannose, D-galactose and N-acetyl-D-glucosamine	Kluyveromyces marxianus

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 28.5fold from strain NBIMCC 1984 by thermal treatment, dialysis, ion exchange chromatography, and chromatofocusing	Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
996.2	-	purified native enzyme	Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 O2.- + 2 H+	-	Kluyveromyces marxianus	O2 + H2O2	-	?
2 O2.- + 2 H+	-	Kluyveromyces marxianus NBIMCC 1984	O2 + H2O2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 34034, sequence calculation and mass spectrometry	Kluyveromyces marxianus

Synonyms

Synonyms	Comment	Organism
Cu/Zn SOD	-	Kluyveromyces marxianus
Cu/Zn superoxide dismutase	-	Kluyveromyces marxianus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Kluyveromyces marxianus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
15	90	temperature stability profile, overview	Kluyveromyces marxianus
60	70	the purified enzyme is quite stable	Kluyveromyces marxianus
75	-	purified enzyme, half-life is 22 min	Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Kluyveromyces marxianus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6.5	8	rapid inactivation below pH 6.5 and above pH 8.0, 60% remaiining activity at pH 7.8 after 10 min at 25°C	Kluyveromyces marxianus

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Release 2023.1 (January 2023)