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Literature summary for 1.15.1.1 extracted from

  • Seo, S.N.; Lee, J.H.; Kim, Y.M.
    Characterization of an iron- and manganese-containing superoxide dismutase from Methylobacillus sp. strain SK1 DSM 8269 (2007), Mol. Cells, 23, 370-378.
    View publication on PubMed
Search for more literature for 1.15.1.1

Inhibitors

Inhibitors Comment Organism Structure
H2O2 H2O2 does not show significant inhibition of the SOD activity in cell-free extracts prepared from cells grown in Mn-rich medium, but inhibits 30% of the enzyme activity in cell extracts from cells grown in Fe-rich medium Methylobacillus sp.
NaN3 inactivates 15% and 24% of the SODs present in cell extracts prepared from cells grown in the Mn- and Fe-rich media, respectively Methylobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a Fe,Mn-SOD, the purified enzyme contains 1.1 g-atom of Fe per mol enzyme Methylobacillus sp.
Mn2+ a Fe,Mn-SOD, the purified enzyme contains 0.7 g-atom of Mn per mol enzyme Methylobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23100
-
 2 * 23100, SDS-PAGE Methylobacillus sp.
44000
-
 gel filtration Methylobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O2.- + H+ Methylobacillus sp.
-
 O2 + H2O2
-
 ?
O2.- + H+ Methylobacillus sp. SK1 / DSM 8269
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Methylobacillus sp.
-
-
-
Methylobacillus sp. SK1 / DSM 8269
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 61.5fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, hydrophobic interaction chromatography and again gel filtration, to homogeneity Methylobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified native enzyme Methylobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2.- + H+
-
 Methylobacillus sp. O2 + H2O2
-
 ?
O2.- + H+
-
 Methylobacillus sp. SK1 / DSM 8269 O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23100, SDS-PAGE Methylobacillus sp.

Synonyms

Synonyms Comment Organism
Fe/MnSOD
-
 Methylobacillus sp.
iron-containing superoxide dismutase
-
 Methylobacillus sp.
manganese-containing superoxide dismutase
-
 Methylobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, purified enzyme, completely stable Methylobacillus sp.
60
-
 30 min, purified enzyme, 73% remaining activity Methylobacillus sp.
70
-
 10 min, purified enzyme, 46% remaining activity, inactivation after 20 min Methylobacillus sp.
80
-
 10 min, purified enzyme, 13% remaining activity, inactivation after 15 min Methylobacillus sp.
90
-
 5 min, purified enzyme, inactivation Methylobacillus sp.
100
-
 4 min, purified enzyme, inactivation Methylobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methylobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Methylobacillus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Methylobacillus sp. isoelectric focusing
-
 4.4