Literature summary for 1.15.1.1 extracted from

De Vendittis, E.; Ursby, T.; Rullo, R.; Gogliettino, M.A.; Masullo, M.; Bocchini, V.
Phenylmethanesulfonyl fluoride inactivates an archaeal superoxide dismutase by chemical modification of a specific tyrosine residue. Cloning, sequencing and expression of the gene coding for Sulfolobus solfataricus superoxide dismutase (2001), Eur. J. Biochem., 268, 1794-1801.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene sod, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109(DE3)	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
phenylmethanesulfonyl fluoride	irreversible inactivation by attachment of a molecule phenylmethanesulfonyl fluoride to the active site Tyr41 reinforcing the heat stability of the enzyme, overview	Saccharolobus solfataricus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	Fe-type SOD	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	4 * 24000, recombinant enzyme, SDS-PAGE	Saccharolobus solfataricus
88000	-	recombinant enzyme, gel filtration	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P80857	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme, and recombinant enzyme from Escherichia coli strain JM109(DE3)	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	native and recombinant enzyme ossess a covalent modification of the conserved Tyr41 in the active site, Tyr41 plays an important role in the enzyme activity and the maintenance of the structural architecture of SOD, overview	Saccharolobus solfataricus	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 24000, recombinant enzyme, SDS-PAGE	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
SOD	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	phenylmethanesulfonyl fluoride attachment to the active site Tyr41 increases the heat stability of the enzyme, overview	Saccharolobus solfataricus

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Release 2023.1 (January 2023)