Application | Comment | Organism |
---|---|---|
synthesis | coexpression of enzyme and variants with yeast copper chaperone yCCS in Escherichia coli, in medium supplemented with copper and zinc, with high yield and activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D90A | mutant related to amyothrophic lateral sclerosis, improvement of expression by coexpression with yeast copper chaperone | Homo sapiens |
F50E/G51E | about 20% of wild-type activity, monomeric | Homo sapiens |
G93A | mutant related to amyothrophic lateral sclerosis, improvement of expression by coexpression with yeast copper chaperone | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | coexpression with yeast copper chaperone, copper supplement of medium, about 1 atom per subunit | Homo sapiens | |
Zinc | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15800 | - |
x * 15800, MALDI-TOF-MS | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
CuZn-type enzyme, expression in Escherichia coli | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 15800, MALDI-TOF-MS | Homo sapiens |