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Literature summary for 1.15.1.1 extracted from

  • Ahl, I.M.; Lindberg, M.J.; Tibell, L.A.E.
    Coexpression of yeast copper chaperone (yCCS) and CuZn-superoxide dismutases in Escherichia coli yields protein with high copper contents (2004), Protein Expr. Purif., 37, 311-319.
    View publication on PubMed

Application

Application Comment Organism
synthesis coexpression of enzyme and variants with yeast copper chaperone yCCS in Escherichia coli, in medium supplemented with copper and zinc, with high yield and activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
D90A mutant related to amyothrophic lateral sclerosis, improvement of expression by coexpression with yeast copper chaperone Homo sapiens
F50E/G51E about 20% of wild-type activity, monomeric Homo sapiens
G93A mutant related to amyothrophic lateral sclerosis, improvement of expression by coexpression with yeast copper chaperone Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper coexpression with yeast copper chaperone, copper supplement of medium, about 1 atom per subunit Homo sapiens
Zinc
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15800
-
x * 15800, MALDI-TOF-MS Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
CuZn-type enzyme, expression in Escherichia coli
-

Subunits

Subunits Comment Organism
? x * 15800, MALDI-TOF-MS Homo sapiens