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Literature summary for 1.15.1.1 extracted from

  • Santos, R.; Bocquet, S.; Puppo, A.; Touati, D.
    Characterization of an atypical superoxide dismutase from Sinorhizobium meliloti (1999), J. Bacteriol., 181, 4509-4516.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
atypical SOD, functional and structural intermediate between Fe-SOD and Mn-SOD, expression in Escherichia coli Sinorhizobium meliloti

Inhibitors

Inhibitors Comment Organism Structure
H2O2 no inhibition: Fe-SOD; no inhibition: Mn-SOD Sinorhizobium meliloti

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 0.24 mol Fe2+ per mol of subunit Sinorhizobium meliloti
Mn2+ 0.75 mol per mol of subunit Sinorhizobium meliloti
Mn2+ accepts iron and/or manganese as cofactor Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000, Fe-/Mn-SOD, SDS-PAGE Sinorhizobium meliloti
43000
-
 Fe-/Mn-SOD, gel filtration Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
-
-
Sinorhizobium meliloti
-
 structural intermediate between Mn-SOD and Fe-SOD
-
Sinorhizobium meliloti Rm5000
-
-
-

Purification (Commentary)

Purification (Comment) Organism
structural intermediate between Mn-SOD and Fe-SOD Sinorhizobium meliloti

Reaction

Reaction Comment Organism Reaction ID
2 superoxide + 2 H+ = O2 + H2O2 amino acid sequence alignment and comparison Sinorhizobium meliloti
a

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of active protein after withdrawal of metal, higher activity with Mn2+, lower activity with Fe3+ Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 native and recombinant, wild-type and restored enzyme, with Fe2+ and Mn2+ Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
 Sinorhizobium meliloti O2 + H2O2
-
 ?
O2- + H+
-
 Sinorhizobium meliloti Rm5000 O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, Fe-/Mn-SOD, SDS-PAGE Sinorhizobium meliloti