Cloned (Comment) | Organism |
---|---|
Mn-SOD, expression in Escherichia coli as His-tagged protein, DNA sequence analysis | Candida albicans |
overexpression in Saccharomyces cerevisiae mutant lacking Cu,Zn-SOD, restores activity of the mutant | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | slight inhibition, Mn-SOD | Candida albicans | |
H2O2 | no inhibition: Mn-SOD | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | SOD-I: Mn-SOD | Candida albicans | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 0.4 mol per mol of mn-SOD | Candida albicans | |
Mn2+ | 0.9 Mn per mol of enzyme | Candida albicans | |
additional information | Mn-SOD contains as well Fe3+, but is only active with manganese | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25400 | - |
4 * 25400, Mn-SOD, SDS-PAGE | Candida albicans |
85000 | - |
recombinant His-tagged Mn-SOD, gel filtration | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | Candida albicans | Mn-SOD, expression is strongly stimulated during stationary phase in cell culture, enzyme is atypical and plays an important role in cell protection against reactive oxygen in the cytosol in the stationary phase | O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q96UT6 | SOD-3 gene, Mn-SOD | - |
Purification (Comment) | Organism |
---|---|
Mn-SOD recombinant from Escherichia coli | Candida albicans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 superoxide + 2 H+ = O2 + H2O2 | amino acid sequence alignment and comparison | Candida albicans |
Renatured (Comment) | Organism |
---|---|
reconstitution of active protein after withdrawal of metal, higher activity with Mn2+, lower activity with Fe3+ | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Candida albicans | O2 + H2O2 | - |
? | |
O2- + H+ | Mn-SOD, expression is strongly stimulated during stationary phase in cell culture, enzyme is atypical and plays an important role in cell protection against reactive oxygen in the cytosol in the stationary phase | Candida albicans | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25400, Mn-SOD, SDS-PAGE | Candida albicans |