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Literature summary for 1.14.99.B10 extracted from

  • Courtade, G.; Wimmer, R.; R?hr, A.K.; Preims, M.; Felice, A.K.; Dimarogona, M.; Vaaje-Kolstad, G.; Sorlie, M.; Sandgren, M.; Ludwig, R.; Eijsink, V.G.; Aachmann, F.L.
    Interactions of a fungal lytic polysaccharide monooxygenase with beta-glucan substrates and cellobiose dehydrogenase (2016), Proc. Natl. Acad. Sci. USA, 113, 5922-5927.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.B10

Activating Compound

Activating Compound Comment Organism Structure
cyanide substrate binding is enhanced for metal-loaded enzymes that are supplied with cyanide Neurospora crassa

Crystallization (Commentary)

Crystallization (Comment) Organism
docking studies with cellulose hexamer. The surface patch surrounding the copper site appears to be a preferred interaction surface. Residue Tyr204 appears not to be involved in binding of this substrate Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the copper ion alone hardly contributes to affinity, but substrate binding is enhanced for metal-loaded enzymes that are supplied with cyanide Neurospora crassa
Zn2+ Zn2+-shows slightly weaker binding compared with Cu2+ Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7SHI8
-
-
Neurospora crassa DSM 1257 Q7SHI8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose hexasaccharide + acceptor + O2
-
 Neurospora crassa ? + reduced acceptor + H2O
-
 ?
cellulose hexasaccharide + acceptor + O2
-
 Neurospora crassa DSM 1257 ? + reduced acceptor + H2O
-
 ?
additional information substrate binding has a substantial effect on the chemical shifts of residues His1, Ala80, His83 and His155 Neurospora crassa ?
-
 ?
additional information substrate binding has a substantial effect on the chemical shifts of residues His1, Ala80, His83 and His155 Neurospora crassa DSM 1257 ?
-
 ?
polymeric xyloglucan + acceptor + O2
-
 Neurospora crassa ? + reduced acceptor + H2O
-
 ?
polymeric xyloglucan + acceptor + O2
-
 Neurospora crassa DSM 1257 ? + reduced acceptor + H2O
-
 ?
xyloglucan 14-mer + acceptor + O2
-
 Neurospora crassa ? + reduced acceptor + H2O
-
 ?
xyloglucan 14-mer + acceptor + O2
-
 Neurospora crassa DSM 1257 ? + reduced acceptor + H2O
-
 ?

General Information

General Information Comment Organism
physiological function enzyme acts in synergy with cellobiose dehydrogenase. The cytochrome domain of cellobiose dehydrogenase interacts with the copper site of the LPMO and substrate binding precludes interaction with cellobiose dehydrogenase Neurospora crassa