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Literature summary for 1.14.99.B10 extracted from

  • Borisova, A.S.; Isaksen, T.; Dimarogona, M.; Kognole; A.A.; Mathiesen, G.; Varnai, A.; Rohr, A.K.; Payne, C.M.; Sorlie, M.; Sandgren, M.; Eijsink, V.G.
    Structural and functional characterization of a lytic polysaccharide monooxygenase with broad substrate specificity (2015), J. Biol. Chem., 290, 22955-22969.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Neurospora crassa

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals grown in presence of Zn2+ and of Cu2+. Structure of the catalytic domain reveals an extended, highly polar substrate-binding surface well suited to interact with a variety of sugar substrates. Binding affinities are in the low micromolar range for polymeric substrates due in part to the presence of a carbohydrate-binding module Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the Cu+ center environment is altered upon substrate binding. The oxidative regioselectivity of LPMO9 enzymes (C1, C4, or both) correlates with distinct structural features of the copper coordination sphere. In strictly C1-oxidizing LPMO9s, access to the solvent-facing axial coordination position is restricted by a conserved tyrosine residue, whereas access to this same position seems unrestricted in C4-oxidizing LPMO9s Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7SHI8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + ascorbate + O2
-
Neurospora crassa ? + dehydroascorbate + H2O
-
?
beta-glucan + ascorbate + O2
-
Neurospora crassa DSM 1257 ? + dehydroascorbate + H2O
-
?
cello-oligosaccharides + ascorbate + O2 with a degree of polymerization of 5 and 7 Neurospora crassa ? + dehydroascorbate + H2O
-
?
cello-oligosaccharides + ascorbate + O2 with a degree of polymerization of 5 and 7 Neurospora crassa DSM 1257 ? + dehydroascorbate + H2O
-
?
low molecular lignin + ascorbate + O2
-
Neurospora crassa ? + dehydroascorbate + H2O
-
?
additional information enzyme acts both on cellulose and on noncellulose beta-glucans, including cellodextrins and xyloglucan Neurospora crassa ?
-
?
additional information enzyme acts both on cellulose and on noncellulose beta-glucans, including cellodextrins and xyloglucan Neurospora crassa DSM 1257 ?
-
?
thio-linked cello-oligosaccharides + ascorbate + O2 with a degree of polymerization of 2–5 Neurospora crassa ? + dehydroascorbate + H2O
-
?
thio-linked xylopentaose + ascorbate + O2
-
Neurospora crassa ? + dehydroascorbate + H2O
-
?
xylogluco-oligosaccharides + ascorbate + O2
-
Neurospora crassa ? + dehydroascorbate + H2O
-
?

Synonyms

Synonyms Comment Organism
LPMO9C
-
Neurospora crassa