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show all sequences of 1.14.99.64

Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis

Zhong, Y.; Huang, J.; Liu, J.; Li, Y.; Jiang, Y.; Xu, Z.; Sandmann, G.; Chen, F.; J. Exp. Bot. 62, 3659-3669 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; transgenic Arabidopsis thaliana expressing the beta-carotene ketolase from Chlamydomonas reinhardtii develop orange leaves which accumulate astaxanthin up to 2 mg/g dry weight with a 1.8fold increase in total carotenoids. The ketolases with high activity of converting zeaxanthin to astaxanthin triggers transgenic Arabidopsis to accumulate large amounts of astaxanthin in leaves and seeds
Chlamydomonas reinhardtii
expression in Escherichia coli; transgenic Arabidopsis thaliana expressing the beta-carotene ketolase from Chlamydomonas reinhardtii develop orange leaves which accumulate astaxanthin up to 2 mg/g dry weight with a 1.8fold increase in total carotenoids. The ketolases with high activity of converting zeaxanthin to astaxanthin triggers transgenic Arabidopsis to accumulate large amounts of astaxanthin in leaves and seeds
Chromochloris zofingiensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
Chromochloris zofingiensis
the enzyme is involved in biosynthesis of astaxanthin
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
Chlamydomonas reinhardtii
the enzyme is involved in biosynthesis of astaxanthin
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
Chromochloris zofingiensis
the enzyme is involved in biosynthesis of astaxanthin
adonixanthin + 2 acceptor + 3 H2O
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
Chlamydomonas reinhardtii
the enzyme is involved in biosynthesis of astaxanthin
adonixanthin + 2 acceptor + 3 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlamydomonas reinhardtii
Q4VKB4
-
-
Chromochloris zofingiensis
Q5U931
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chromochloris zofingiensis
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chlamydomonas reinhardtii
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme shows high (about 85%) conversion rate from zeaxanthin to astaxanthin
748319
Chlamydomonas reinhardtii
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme shows moderate (about 38%) conversion rate from zeaxanthin to astaxanthin
748319
Chromochloris zofingiensis
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chromochloris zofingiensis
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chlamydomonas reinhardtii
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme shows high (about 85%) conversion rate from zeaxanthin to astaxanthin
748319
Chlamydomonas reinhardtii
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme shows moderate (about 38%) conversion rate from zeaxanthin to astaxanthin
748319
Chromochloris zofingiensis
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; transgenic Arabidopsis thaliana expressing the beta-carotene ketolase from Chlamydomonas reinhardtii develop orange leaves which accumulate astaxanthin up to 2 mg/g dry weight with a 1.8fold increase in total carotenoids. The ketolases with high activity of converting zeaxanthin to astaxanthin triggers transgenic Arabidopsis to accumulate large amounts of astaxanthin in leaves and seeds
Chlamydomonas reinhardtii
expression in Escherichia coli; transgenic Arabidopsis thaliana expressing the beta-carotene ketolase from Chlamydomonas reinhardtii develop orange leaves which accumulate astaxanthin up to 2 mg/g dry weight with a 1.8fold increase in total carotenoids. The ketolases with high activity of converting zeaxanthin to astaxanthin triggers transgenic Arabidopsis to accumulate large amounts of astaxanthin in leaves and seeds
Chromochloris zofingiensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
Chromochloris zofingiensis
the enzyme is involved in biosynthesis of astaxanthin
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
Chlamydomonas reinhardtii
the enzyme is involved in biosynthesis of astaxanthin
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
Chromochloris zofingiensis
the enzyme is involved in biosynthesis of astaxanthin
adonixanthin + 2 acceptor + 3 H2O
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
Chlamydomonas reinhardtii
the enzyme is involved in biosynthesis of astaxanthin
adonixanthin + 2 acceptor + 3 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chromochloris zofingiensis
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chlamydomonas reinhardtii
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme shows high (about 85%) conversion rate from zeaxanthin to astaxanthin
748319
Chlamydomonas reinhardtii
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme shows moderate (about 38%) conversion rate from zeaxanthin to astaxanthin
748319
Chromochloris zofingiensis
(3S,3'S)-astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chromochloris zofingiensis
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in biosynthesis of astaxanthin
748319
Chlamydomonas reinhardtii
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme shows high (about 85%) conversion rate from zeaxanthin to astaxanthin
748319
Chlamydomonas reinhardtii
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
zeaxanthin + 2 reduced acceptor + 2 O2
the enzyme shows moderate (about 38%) conversion rate from zeaxanthin to astaxanthin
748319
Chromochloris zofingiensis
adonixanthin + 2 acceptor + 3 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of astaxanthin
Chlamydomonas reinhardtii
metabolism
the enzyme is involved in biosynthesis of astaxanthin
Chromochloris zofingiensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of astaxanthin
Chlamydomonas reinhardtii
metabolism
the enzyme is involved in biosynthesis of astaxanthin
Chromochloris zofingiensis
Other publictions for EC 1.14.99.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748319
Zhong
Functional characterization o ...
Chlamydomonas reinhardtii, Chromochloris zofingiensis
J. Exp. Bot.
62
3659-3669
2011
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