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Literature summary for 1.14.99.63 extracted from

  • Wang, R.; Gu, X.; Yao, M.; Pan, C.; Liu, H.; Xiao, W.; Wang, Y.; Yuan, Y.
    Engineering of beta-carotene hydroxylase and ketolase for astaxanthin overproduction in Saccharomyces cerevisiae (2017), Front. Chem. Sci. Engin., 11, 89-99 .
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Application

Application Comment Organism
synthesis construction of an astaxanthin biosynthesis pathway in Saccharomyces cerevisiae by introducing heterologous beta-carotene hydroxylase (CrtZ) and beta-carotene ketolase (CrtW) into an existing high beta-carotene producing strain. Astaxanthin yield of 3.1 mg/g dry cell weight are achieved. After change of promoter and hrough high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reaches to 81.0 mg/l Brevundimonas vesicularis

Cloned(Commentary)

Cloned (Comment) Organism
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight Brevundimonas vesicularis
expression in Saccharomyces cerevisiae Brevundimonas vesicularis

Organism

Organism UniProt Comment Textmining
Brevundimonas vesicularis Q0H2C9
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Brevundimonas vesicularis DC263 Q0H2C9
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Synonyms

Synonyms Comment Organism
CrtW
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 Brevundimonas vesicularis