BRENDA - Enzyme Database show
show all sequences of 1.14.99.58

Variable composition of heme oxygenases with different regiospecificities in Pseudomonas species

Gisk, B.; Wiethaus, J.; Aras, M.; Frankenberg-Dinkel, N.; Arch. Microbiol. 194, 597-606 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3); gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas fluorescens
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3); gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas mendocina
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3); gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3); genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of N-terminally His6-tagged BphO in Escherichia coli strain BL21(DE3); gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas syringae pv. tomato
genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3)
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics for PigA, overview
Pseudomonas aeruginosa
additional information
-
additional information
Michaelis-Menten kinetics for PigA, overview
Pseudomonas fluorescens
additional information
-
additional information
Michaelis-Menten kinetics for PigA, overview
Pseudomonas putida
0.0242
-
heme
PigA, pH and temperature not specified in the publication
Pseudomonas fluorescens
0.0269
-
heme
pH and temperature not specified in the publication
Pseudomonas aeruginosa
0.0269
-
heme
pH and temperature not specified in the publication
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
heme + 3 AH2 + 3 O2
Pseudomonas syringae pv. tomato
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas aeruginosa
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas fluorescens
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas mendocina
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas putida
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas aeruginosa
natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas fluorescens Pf-5
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas syringae pv. tomato DC3000
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas aeruginosa KT 2240
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas putida KT 2240
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas mendocina YMP
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
additional information
Pseudomonas aeruginosa
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas putida
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas syringae pv. tomato
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas fluorescens
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas mendocina
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas fluorescens Pf-5
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas syringae pv. tomato DC3000
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas aeruginosa KT 2240
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas putida KT 2240
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas mendocina YMP
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
O69002
PigA; gene pigA
-
Pseudomonas aeruginosa
Q88P48
gene hemO; two genes pigA encoding PigA homologues
-
Pseudomonas aeruginosa
Q9HWR4
BphO; gene bphO
-
Pseudomonas aeruginosa KT 2240
Q88P48
gene hemO; two genes pigA encoding PigA homologues
-
Pseudomonas fluorescens
Q4K657
BphO; gene bphO
-
Pseudomonas fluorescens
Q4K7S1
PigA; gene pigA
-
Pseudomonas fluorescens Pf-5
Q4K657
BphO; gene bphO
-
Pseudomonas fluorescens Pf-5
Q4K7S1
PigA; gene pigA
-
Pseudomonas mendocina
A4Y0Y8
; PigA; gene pigA
-
Pseudomonas mendocina YMP
A4Y0Y8
; PigA; gene pigA
-
Pseudomonas putida
Q88JR7
two genes pigA encoding PigA homologues
-
Pseudomonas putida KT 2240
Q88JR7
two genes pigA encoding PigA homologues
-
Pseudomonas syringae pv. tomato
Q885D4
BphO; pv. tomato, gene bphO
-
Pseudomonas syringae pv. tomato
Q887K9
PigA; pv. tomato, gene pigA
-
Pseudomonas syringae pv. tomato DC3000
Q885D4
BphO; pv. tomato, gene bphO
-
Pseudomonas syringae pv. tomato DC3000
Q887K9
PigA; pv. tomato, gene pigA
-
Purification (Commentary)
Commentary
Organism
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography; recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas fluorescens
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography; recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas mendocina
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography; recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography; recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas aeruginosa
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by affinity chromatography; recombinant N-terminally His6-tagged BphO from Escherichia coli strain BL21(DE3) by affinity chromatography
Pseudomonas syringae pv. tomato
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas syringae pv. tomato
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas fluorescens
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas mendocina
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas putida
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas syringae pv. tomato
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas fluorescens
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas mendocina
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas putida
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ferredoxin from spinach, and spinach ferredoxin-NADP+-oxidoreductase
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas fluorescens Pf-5
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas fluorescens Pf-5
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas syringae pv. tomato DC3000
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas syringae pv. tomato DC3000
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas aeruginosa KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas aeruginosa KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas putida KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas putida KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas mendocina YMP
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas mendocina YMP
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas aeruginosa
?
-
-
-
-
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas putida
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas syringae pv. tomato
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas aeruginosa
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas fluorescens
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas mendocina
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas fluorescens Pf-5
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas syringae pv. tomato DC3000
?
-
-
-
-
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas aeruginosa KT 2240
?
-
-
-
-
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas putida KT 2240
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas mendocina YMP
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
above, PigA; above, PigA
Pseudomonas aeruginosa
45
-
above, PigA
Pseudomonas fluorescens
45
-
above, PigA
Pseudomonas mendocina
45
-
above, PigA
Pseudomonas putida
45
-
above, PigA
Pseudomonas syringae pv. tomato
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
PigA
Pseudomonas fluorescens
6.75
-
PigA
Pseudomonas aeruginosa
6.75
-
PigA
Pseudomonas putida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
high activity of PigA within this range; high activity of PigA within this range
Pseudomonas aeruginosa
6
9
high activity of PigA within this range
Pseudomonas fluorescens
6
9
high activity of PigA within this range
Pseudomonas mendocina
6
9
high activity of PigA within this range
Pseudomonas putida
6
9
high activity of PigA within this range
Pseudomonas syringae pv. tomato
Cofactor
Cofactor
Commentary
Organism
Structure
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas aeruginosa
ferredoxin-NADP+-oxidoreductase
;
Pseudomonas fluorescens
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas mendocina
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas putida
ferredoxin-NADP+-oxidoreductase
;
Pseudomonas syringae pv. tomato
additional information
with PigA, there is no difference in product formation whether ferredoxin-NADP+-oxidoreductase or ferredoxin, FNR and ascorbate are used as electron donor
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3)
Pseudomonas fluorescens
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3); gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas mendocina
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of N-terminally His6-tagged BphO in Escherichia coli strain BL21(DE3)
Pseudomonas syringae pv. tomato
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas fluorescens
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
Pseudomonas syringae pv. tomato
genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3)
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas aeruginosa
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas fluorescens
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas mendocina
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas putida
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas syringae pv. tomato
additional information
with PigA, there is no difference in product formation whether ferredoxin-NADP+-oxidoreductase or ferredoxin, FNR and ascorbate are used as electron donor
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics for PigA, overview
Pseudomonas aeruginosa
additional information
-
additional information
Michaelis-Menten kinetics for PigA, overview
Pseudomonas fluorescens
additional information
-
additional information
Michaelis-Menten kinetics for PigA, overview
Pseudomonas putida
0.0242
-
heme
PigA, pH and temperature not specified in the publication
Pseudomonas fluorescens
0.0269
-
heme
pH and temperature not specified in the publication
Pseudomonas aeruginosa
0.0269
-
heme
pH and temperature not specified in the publication
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
heme + 3 AH2 + 3 O2
Pseudomonas syringae pv. tomato
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas aeruginosa
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas fluorescens
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas mendocina
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas putida
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas aeruginosa
natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas fluorescens Pf-5
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas syringae pv. tomato DC3000
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas aeruginosa KT 2240
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas putida KT 2240
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
heme + 3 AH2 + 3 O2
Pseudomonas mendocina YMP
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
?
additional information
Pseudomonas aeruginosa
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas putida
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas syringae pv. tomato
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas fluorescens
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas mendocina
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas fluorescens Pf-5
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas syringae pv. tomato DC3000
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
additional information
Pseudomonas aeruginosa KT 2240
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas putida KT 2240
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
?
-
-
-
additional information
Pseudomonas mendocina YMP
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas aeruginosa
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas fluorescens
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography; recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas mendocina
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by affinity chromatography
Pseudomonas syringae pv. tomato
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas aeruginosa
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas fluorescens
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Pseudomonas putida
recombinant N-terminally His6-tagged BphO from Escherichia coli strain BL21(DE3) by affinity chromatography
Pseudomonas syringae pv. tomato
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas syringae pv. tomato
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas fluorescens
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas mendocina
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas putida
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas syringae pv. tomato
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas fluorescens
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas mendocina
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas putida
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ferredoxin from spinach, and spinach ferredoxin-NADP+-oxidoreductase
726833
Pseudomonas aeruginosa
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas fluorescens Pf-5
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas fluorescens Pf-5
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas syringae pv. tomato DC3000
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas syringae pv. tomato DC3000
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas aeruginosa KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas aeruginosa KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas putida KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas putida KT 2240
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
-
726833
Pseudomonas mendocina YMP
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
heme + 3 AH2 + 3 O2
with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination
726833
Pseudomonas mendocina YMP
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
-
-
?
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas aeruginosa
?
-
-
-
-
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas putida
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas syringae pv. tomato
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas aeruginosa
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas fluorescens
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas mendocina
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas fluorescens Pf-5
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas syringae pv. tomato DC3000
?
-
-
-
-
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas aeruginosa KT 2240
?
-
-
-
-
additional information
the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha
726833
Pseudomonas putida KT 2240
?
-
-
-
-
additional information
the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta
726833
Pseudomonas mendocina YMP
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
above, PigA
Pseudomonas aeruginosa
45
-
above, PigA
Pseudomonas fluorescens
45
-
above, PigA
Pseudomonas mendocina
45
-
above, PigA
Pseudomonas putida
45
-
above, PigA
Pseudomonas syringae pv. tomato
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
PigA
Pseudomonas fluorescens
6.75
-
PigA
Pseudomonas aeruginosa
6.75
-
PigA
Pseudomonas putida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
high activity of PigA within this range
Pseudomonas aeruginosa
6
9
high activity of PigA within this range
Pseudomonas fluorescens
6
9
high activity of PigA within this range
Pseudomonas mendocina
6
9
high activity of PigA within this range
Pseudomonas putida
6
9
high activity of PigA within this range
Pseudomonas syringae pv. tomato
Other publictions for EC 1.14.99.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742894
Mourino
Metabolite-driven regulation ...
Pseudomonas aeruginosa
J. Biol. Chem.
291
20503-20515
2016
-
-
1
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726833
Gisk
Variable composition of heme o ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa KT 2240, Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5, Pseudomonas mendocina, Pseudomonas mendocina YMP, Pseudomonas putida, Pseudomonas putida KT 2240, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Arch. Microbiol.
194
597-606
2012
-
-
5
-
-
-
-
6
-
-
-
30
-
16
-
-
5
-
-
-
-
-
48
-
5
-
-
-
3
5
-
6
-
-
-
-
-
9
10
-
-
-
-
-
-
6
-
-
-
30
-
-
-
9
-
-
-
-
48
-
6
-
-
-
3
6
-
-
-
-
-
-
-
-
742839
Barker
Metabolic flux of extracellul ...
Pseudomonas aeruginosa
J. Biol. Chem.
287
18342-18350
2012
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
692400
Barkovits
Expression of the phytochrome ...
Pseudomonas aeruginosa
FEMS Microbiol. Lett.
80
160-168
2008
1
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
680357
Rodríguez
The hydrogen-bonding network i ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
129
11730-11742
2007
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681311
Furci
Inhibition of the bacterial he ...
Pseudomonas aeruginosa
J. Med. Chem.
50
3804-3813
2007
-
1
-
-
-
-
13
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
13
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678146
Bhakta
The mechanism of heme transfer ...
Pseudomonas aeruginosa
Biochemistry
45
11642-11649
2006
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678184
Rodríguez
Backbone NMR assignments and H ...
Pseudomonas aeruginosa
Biochemistry
45
4578-4592
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659407
Wegele
The heme oxygenase(s)-phytochr ...
Pseudomonas aeruginosa
J. Biol. Chem.
279
45791-45802
2004
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742737
Fujii
Essential amino acid residues ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
126
4466-4467
2004
-
-
1
-
8
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661923
Caignan
Oxidation of heme to beta- and ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
124
14879-14892
2002
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742771
Ratliff
Homologues of neisserial heme ...
Pseudomonas aeruginosa
J. Bacteriol.
183
6394-6403
2001
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-