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Literature summary for 1.14.99.58 extracted from

  • Gisk, B.; Wiethaus, J.; Aras, M.; Frankenberg-Dinkel, N.
    Variable composition of heme oxygenases with different regiospecificities in Pseudomonas species (2012), Arch. Microbiol., 194, 597-606.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3) Pseudomonas mendocina
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of GST-tagged BphO in Escherichia coli strain BL21(DE3) Pseudomonas fluorescens
gene bphO, BphO is genetically coupled to the phytochrome BphP, expression of N-terminally His6-tagged BphO in Escherichia coli strain BL21(DE3) Pseudomonas syringae pv. tomato
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) Pseudomonas fluorescens
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) Pseudomonas mendocina
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3) Pseudomonas syringae pv. tomato
genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa
genes pigA, two homologues, one of the two PigA homologues identified in Pseudomonas putida KT2440 is encoded in an iron-associated gene cluster, expression of GST-tagged PigA and BphO in Escherichia coli strain BL21(DE3) Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics for PigA, overview Pseudomonas aeruginosa
additional information
-
additional information Michaelis-Menten kinetics for PigA, overview Pseudomonas fluorescens
additional information
-
additional information Michaelis-Menten kinetics for PigA, overview Pseudomonas putida
0.0242
-
heme PigA, pH and temperature not specified in the publication Pseudomonas fluorescens
0.0269
-
heme pH and temperature not specified in the publication Pseudomonas aeruginosa
0.0269
-
heme pH and temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heme + 3 AH2 + 3 O2 Pseudomonas syringae pv. tomato
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas aeruginosa
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas fluorescens
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas mendocina
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas putida
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas aeruginosa natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas fluorescens Pf-5
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas syringae pv. tomato DC3000
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas aeruginosa KT 2240
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas putida KT 2240
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 Pseudomonas mendocina YMP
-
biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
additional information Pseudomonas aeruginosa the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha ?
-
?
additional information Pseudomonas putida the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha ?
-
?
additional information Pseudomonas syringae pv. tomato the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta ?
-
?
additional information Pseudomonas fluorescens the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta ?
-
?
additional information Pseudomonas mendocina the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta ?
-
?
additional information Pseudomonas fluorescens Pf-5 the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta ?
-
?
additional information Pseudomonas syringae pv. tomato DC3000 the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta ?
-
?
additional information Pseudomonas aeruginosa KT 2240 the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha ?
-
?
additional information Pseudomonas putida KT 2240 the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha ?
-
?
additional information Pseudomonas mendocina YMP the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa O69002 PigA; gene pigA
-
Pseudomonas aeruginosa Q88P48 gene hemO; two genes pigA encoding PigA homologues
-
Pseudomonas aeruginosa Q9HWR4 BphO; gene bphO
-
Pseudomonas aeruginosa KT 2240 Q88P48 gene hemO; two genes pigA encoding PigA homologues
-
Pseudomonas fluorescens Q4K657 BphO; gene bphO
-
Pseudomonas fluorescens Q4K7S1 PigA; gene pigA
-
Pseudomonas fluorescens Pf-5 Q4K657 BphO; gene bphO
-
Pseudomonas fluorescens Pf-5 Q4K7S1 PigA; gene pigA
-
Pseudomonas mendocina A4Y0Y8
-
-
Pseudomonas mendocina A4Y0Y8 PigA; gene pigA
-
Pseudomonas mendocina YMP A4Y0Y8
-
-
Pseudomonas mendocina YMP A4Y0Y8 PigA; gene pigA
-
Pseudomonas putida Q88JR7 two genes pigA encoding PigA homologues
-
Pseudomonas putida KT 2240 Q88JR7 two genes pigA encoding PigA homologues
-
Pseudomonas syringae pv. tomato Q885D4 BphO; pv. tomato, gene bphO
-
Pseudomonas syringae pv. tomato Q887K9 PigA; pv. tomato, gene pigA
-
Pseudomonas syringae pv. tomato DC3000 Q885D4 BphO; pv. tomato, gene bphO
-
Pseudomonas syringae pv. tomato DC3000 Q887K9 PigA; pv. tomato, gene pigA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas mendocina
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas aeruginosa
recombinant GST-tagged BphO from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas fluorescens
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by affinity chromatography Pseudomonas syringae pv. tomato
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas aeruginosa
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas fluorescens
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas mendocina
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Pseudomonas putida
recombinant N-terminally His6-tagged BphO from Escherichia coli strain BL21(DE3) by affinity chromatography Pseudomonas syringae pv. tomato

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heme + 3 AH2 + 3 O2
-
Pseudomonas syringae pv. tomato biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas aeruginosa biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas fluorescens biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas mendocina biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas putida biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase Pseudomonas aeruginosa biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas syringae pv. tomato biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas aeruginosa biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas fluorescens biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas mendocina biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas putida biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ferredoxin from spinach, and spinach ferredoxin-NADP+-oxidoreductase Pseudomonas aeruginosa biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas fluorescens Pf-5 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas fluorescens Pf-5 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas syringae pv. tomato DC3000 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas syringae pv. tomato DC3000 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas aeruginosa KT 2240 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas aeruginosa KT 2240 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas putida KT 2240 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas putida KT 2240 biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2
-
Pseudomonas mendocina YMP biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
heme + 3 AH2 + 3 O2 with NADPH, ascorbate, FNR and ferredoxin as exogenous reductant system, spectroscopic determination Pseudomonas mendocina YMP biliverdin + Fe2+ + CO + 3 A + 3 H2O
-
?
additional information the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha Pseudomonas aeruginosa ?
-
?
additional information the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha Pseudomonas putida ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas syringae pv. tomato ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas aeruginosa ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas fluorescens ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas mendocina ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas fluorescens Pf-5 ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas syringae pv. tomato DC3000 ?
-
?
additional information the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha Pseudomonas aeruginosa KT 2240 ?
-
?
additional information the organism contains only one enzyme type: PigA. PigA produces the biliverdin isomer IXalpha Pseudomonas putida KT 2240 ?
-
?
additional information the organism contains two heme oxygenases with different regiospecificities: BphO and PigA. While BphO cleaves heme to exclusively yield biliverdin IXalpha, PigA produces the biliverdin isomers IXbeta and IXdelta Pseudomonas mendocina YMP ?
-
?

Synonyms

Synonyms Comment Organism
BphO
-
Pseudomonas syringae pv. tomato
BphO
-
Pseudomonas mendocina
BphO
-
Pseudomonas aeruginosa
BphO
-
Pseudomonas fluorescens
PigA
-
Pseudomonas aeruginosa
PigA
-
Pseudomonas fluorescens
PigA
-
Pseudomonas mendocina
PigA
-
Pseudomonas syringae pv. tomato
PigA
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
above, PigA Pseudomonas aeruginosa
45
-
above, PigA Pseudomonas fluorescens
45
-
above, PigA Pseudomonas mendocina
45
-
above, PigA Pseudomonas syringae pv. tomato
45
-
above, PigA Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
PigA Pseudomonas fluorescens
6.75
-
PigA Pseudomonas aeruginosa
6.75
-
PigA Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 9 high activity of PigA within this range Pseudomonas aeruginosa
6 9 high activity of PigA within this range Pseudomonas fluorescens
6 9 high activity of PigA within this range Pseudomonas mendocina
6 9 high activity of PigA within this range Pseudomonas syringae pv. tomato
6 9 high activity of PigA within this range Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas syringae pv. tomato
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas aeruginosa
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas fluorescens
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas mendocina
ferredoxin-NADP+-oxidoreductase
-
Pseudomonas putida
additional information with PigA, there is no difference in product formation whether ferredoxin-NADP+-oxidoreductase or ferredoxin, FNR and ascorbate are used as electron donor Pseudomonas aeruginosa