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Backbone NMR assignments and H/D exchange studies on the ferric azide- and cyanide-inhibited forms of Pseudomonas aeruginosa heme oxygenase

Rodríguez, J.C.; Wilks, A.; Rivera, M.; Biochemistry 45, 4578-4592 (2006)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
NMR-spectroscopy studies of paramagnetic cyanide-inhibited and azide-inhibited enzyme forms. The protein in the azide-inhibited complex is significantly less prone to H/D exchange than the cyanide-inhibited form. Unpaired spin delocalization from the heme iron into the G15-N atom via formation of a hydrogen bond between the coordinated azide nitrogen and the G125 N-H
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
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Crystallization (Commentary) (protein specific)
Crystallization
Organism
NMR-spectroscopy studies of paramagnetic cyanide-inhibited and azide-inhibited enzyme forms. The protein in the azide-inhibited complex is significantly less prone to H/D exchange than the cyanide-inhibited form. Unpaired spin delocalization from the heme iron into the G15-N atom via formation of a hydrogen bond between the coordinated azide nitrogen and the G125 N-H
Pseudomonas aeruginosa
Other publictions for EC 1.14.99.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742894
Mourino
Metabolite-driven regulation ...
Pseudomonas aeruginosa
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726833
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Variable composition of heme o ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa KT 2240, Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5, Pseudomonas mendocina, Pseudomonas mendocina YMP, Pseudomonas putida, Pseudomonas putida KT 2240, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
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30
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742839
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Metabolic flux of extracellul ...
Pseudomonas aeruginosa
J. Biol. Chem.
287
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2012
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692400
Barkovits
Expression of the phytochrome ...
Pseudomonas aeruginosa
FEMS Microbiol. Lett.
80
160-168
2008
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680357
Rodríguez
The hydrogen-bonding network i ...
Pseudomonas aeruginosa
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129
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2007
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1
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681311
Furci
Inhibition of the bacterial he ...
Pseudomonas aeruginosa
J. Med. Chem.
50
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2007
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1
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678146
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The mechanism of heme transfer ...
Pseudomonas aeruginosa
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45
11642-11649
2006
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4
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678184
Rodríguez
Backbone NMR assignments and H ...
Pseudomonas aeruginosa
Biochemistry
45
4578-4592
2006
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Pseudomonas aeruginosa
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279
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2004
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742737
Fujii
Essential amino acid residues ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
126
4466-4467
2004
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661923
Caignan
Oxidation of heme to beta- and ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
124
14879-14892
2002
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742771
Ratliff
Homologues of neisserial heme ...
Pseudomonas aeruginosa
J. Bacteriol.
183
6394-6403
2001
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