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show all sequences of 1.14.99.58

Oxidation of heme to beta- and delta-biliverdin by Pseudomonas aeruginosa heme oxygenase as a consequence of an unusual seating of the heme

Caignan, G.A.; Deshmukh, R.; Wilks, A.; Zeng, Y.; Huang, H.W.; Moenne-Loccoz, P.; Bunce, R.A.; Eastman, M.A.; Rivera, M.; J. Am. Chem. Soc. 124, 14879-14892 (2002)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
F117Y
no change in regioselectivity
Pseudomonas aeruginosa
N19K
no change in regioselectivity
Pseudomonas aeruginosa
N19K/F117Y
change in regioselectivity, producing alpha-biliverdin and less beta- and delta-biliverdin, enzyme exists as a mixture of molecules exhibiting 2 distinct heme seatings, one seating is identical to wild-type, the other is similar to that typical of alpha-hydroxylating heme oxigenases
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
heme + electron donor + O2
-
661923
Pseudomonas aeruginosa
alpha-biliverdin + beta-biliverdin + delta-biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
wild-type, mutant N19K, mutant F117Y, 30% beta- and 70% delta-isomer, N19K/F117Y double mutant, 55% alpha-, 10% beta-, 35% delta-isoform
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F117Y
no change in regioselectivity
Pseudomonas aeruginosa
N19K
no change in regioselectivity
Pseudomonas aeruginosa
N19K/F117Y
change in regioselectivity, producing alpha-biliverdin and less beta- and delta-biliverdin, enzyme exists as a mixture of molecules exhibiting 2 distinct heme seatings, one seating is identical to wild-type, the other is similar to that typical of alpha-hydroxylating heme oxigenases
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
heme + electron donor + O2
-
661923
Pseudomonas aeruginosa
alpha-biliverdin + beta-biliverdin + delta-biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
wild-type, mutant N19K, mutant F117Y, 30% beta- and 70% delta-isomer, N19K/F117Y double mutant, 55% alpha-, 10% beta-, 35% delta-isoform
-
-
?
Other publictions for EC 1.14.99.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742894
Mourino
Metabolite-driven regulation ...
Pseudomonas aeruginosa
J. Biol. Chem.
291
20503-20515
2016
-
-
1
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2
-
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-
-
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2
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1
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2
1
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1
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2
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2
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2
1
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726833
Gisk
Variable composition of heme o ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa KT 2240, Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5, Pseudomonas mendocina, Pseudomonas mendocina YMP, Pseudomonas putida, Pseudomonas putida KT 2240, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Arch. Microbiol.
194
597-606
2012
-
-
5
-
-
-
-
6
-
-
-
30
-
16
-
-
5
-
-
-
-
-
48
-
5
-
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-
3
5
-
6
-
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-
9
10
-
-
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-
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-
6
-
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-
30
-
-
-
9
-
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-
48
-
6
-
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-
3
6
-
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742839
Barker
Metabolic flux of extracellul ...
Pseudomonas aeruginosa
J. Biol. Chem.
287
18342-18350
2012
-
-
1
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2
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1
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2
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2
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-
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-
1
1
-
-
-
692400
Barkovits
Expression of the phytochrome ...
Pseudomonas aeruginosa
FEMS Microbiol. Lett.
80
160-168
2008
1
-
1
-
1
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-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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680357
Rodríguez
The hydrogen-bonding network i ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
129
11730-11742
2007
-
-
-
1
1
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-
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1
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1
1
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681311
Furci
Inhibition of the bacterial he ...
Pseudomonas aeruginosa
J. Med. Chem.
50
3804-3813
2007
-
1
-
-
-
-
13
-
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1
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1
-
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1
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13
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1
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678146
Bhakta
The mechanism of heme transfer ...
Pseudomonas aeruginosa
Biochemistry
45
11642-11649
2006
-
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-
-
4
-
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-
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-
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1
-
1
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4
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1
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678184
Rodríguez
Backbone NMR assignments and H ...
Pseudomonas aeruginosa
Biochemistry
45
4578-4592
2006
-
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1
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1
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659407
Wegele
The heme oxygenase(s)-phytochr ...
Pseudomonas aeruginosa
J. Biol. Chem.
279
45791-45802
2004
-
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1
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2
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1
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1
1
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1
1
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1
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2
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1
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1
1
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742737
Fujii
Essential amino acid residues ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
126
4466-4467
2004
-
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1
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8
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2
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1
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2
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8
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2
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661923
Caignan
Oxidation of heme to beta- and ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
124
14879-14892
2002
-
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3
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1
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3
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742771
Ratliff
Homologues of neisserial heme ...
Pseudomonas aeruginosa
J. Bacteriol.
183
6394-6403
2001
-
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1
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2
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1
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1
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1
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1
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1
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