Literature summary for 1.14.99.54 extracted from

Tanghe, M.; Danneels, B.; Last, M.; Beerens, K.; Stals, I.; Desmet, T.
Disulfide bridges as essential elements for the thermostability of lytic polysaccharide monooxygenase LPMO10C from Streptomyces coelicolor (2017), Protein Eng. Des. Sel., 30, 401-408 .

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Protein Variants

Protein Variants	Comment	Organism
A143C/P183C/S73C/A115C	introduction of addtitional disulfide bridges A143C-P183C and S73C-A115C, increase in melting temperature by 12 degrees	Streptomyces coelicolor

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	Q9RJY2	-	-
Streptomyces coelicolor ATCC BAA-471	Q9RJY2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoric acid swollen cellulose + ascorbic acid + O2	-	Streptomyces coelicolor	cellobionic acid + ? + dehydroascorbate + H2O	-	?
phosphoric acid swollen cellulose + ascorbic acid + O2	-	Streptomyces coelicolor ATCC BAA-471	cellobionic acid + ? + dehydroascorbate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
LPMO10C	-	Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
51	-	melting temperature, wild-type	Streptomyces coelicolor
63	-	melting temperature, mutant A143C/P183C/S73C/A115C	Streptomyces coelicolor
80	-	2 h, 34% residual activity for wild-type	Streptomyces coelicolor

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Release 2023.1 (January 2023)