Application | Comment | Organism |
---|---|---|
analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Thermothelomyces thermophilus |
analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Thermothielavioides terrestris |
analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Trichoderma reesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, complete loss of activity | Thermothielavioides terrestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | EDTA-inhibited enzyme can be reactivated by 20 mM Cu2+ within 1 h | Thermothielavioides terrestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | A0A0H4K9X4 | - |
- |
Thermothielavioides terrestris | D0VWZ9 | - |
- |
Trichoderma reesei | O14405 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + ascorbic acid + O2 | - |
Thermothelomyces thermophilus | ? + dehydroascorbate + H2O | - |
? | |
cellulose + ascorbic acid + O2 | - |
Thermothielavioides terrestris | ? + dehydroascorbate + H2O | - |
? | |
cellulose + ascorbic acid + O2 | - |
Trichoderma reesei | ? + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel61a | - |
Trichoderma reesei |
gh61e | - |
Thermothielavioides terrestris |
LPMO9A | - |
Thermothelomyces thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
cellulose | pH 5.0, 37°C | Thermothielavioides terrestris | |
0.021 | - |
cellulose | pH 5.0, 37°C | Thermothelomyces thermophilus | |
0.021 | - |
cellulose | pH 5.0, 37°C | Trichoderma reesei |