Application | Comment | Organism |
---|---|---|
degradation | design of dockerin-fused lytic polysaccharide monooxygenases. The resulting chimeras exhibit activity levels on microcrystalline cellulose similar to that of the wild-type enzymes. The dockerin moieties of the chimeras are functional and specifically bind to their corresponding cohesin partner. The chimeric lytic polysaccharide monooxygenases are able to self-assemble in designer cellulosomes alongside an endo- and an exo-cellulase also converted to the cellulosomal mode. The resulting complexes show a 1.7fold increase in the release of soluble sugars from cellulose, compared with the free enzymes, and a 2.6fold enhancement compared with free cellulases without lytic polysaccharide monooxygenase enhancement | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | Q47PB9 | - |
- |
Thermobifida fusca | Q47QG3 | enzyme has both C1-hydroxylating and C4-dehydrogenating activities | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
microcrystalline cellulose + AH2 + O2 | - |
Thermobifida fusca | ? | enzyme catalyzes release of a mixture of soluble sugars comprising reduced and oxidized cellooligosaccharides. The degree of polymerization of the released oligosaccharides ranges from 3 to 5 for the reduced products and from 2 to 5 for the oxidized products | ? |
Synonyms | Comment | Organism |
---|---|---|
LPMO10A | - |
Thermobifida fusca |
LPMO10B | - |
Thermobifida fusca |
Tfu_1268 | - |
Thermobifida fusca |
Tfu_1665 | - |
Thermobifida fusca |