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Literature summary for 1.14.99.54 extracted from

  • Frommhagen, M; Sforza, S.; Westphal, A.H.; Visser, J.; Hinz, S.W.; Koetsier, M.J.; van Berkel, W.J.; Gruppen, H.; Kabel, M.A.
    Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase (2015), Biotechnol. Biofuels, 17, 101.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.54

Cloned(Commentary)

Cloned (Comment) Organism
expressed and produced in a protease/(hemi-) cellulase-free Myceliophthora thermophila C1 strain Thermothelomyces thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22770
-
 mass spectrometry Thermothelomyces thermophilus
23000
-
 SDS-PAGE Thermothelomyces thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermothelomyces thermophilus The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone ?
-
 ?
additional information Thermothelomyces thermophilus C1 The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone ?
-
 ?
[(1->4)-beta-D-glucosyl]n+m + AH2 + O2 Thermothelomyces thermophilus
-
 [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?
[(1->4)-beta-D-glucosyl]n+m + AH2 + O2 Thermothelomyces thermophilus C1
-
 [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus A0A0H4K9X4 Thielavia heterothallica
-
Thermothelomyces thermophilus C1 A0A0H4K9X4 Thielavia heterothallica
-

Posttranslational Modification

Posttranslational Modification Comment Organism
methylation the enzyme contains an N-methylated histidine at the N-terminus Thermothelomyces thermophilus

Purification (Commentary)

Purification (Comment) Organism
-
 Thermothelomyces thermophilus

Reaction

Reaction Comment Organism Reaction ID
[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + acceptor + H2O (1) the D-glucono-1,5-lactone at the reducing end of the cellulose chain quickly hydrolyzes spontaneuosly to the aldonic acid Thermothelomyces thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone Thermothelomyces thermophilus ?
-
 ?
additional information The enzyme also oxidizes wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan if assayed in the presence of amorphous cellulose. The enzyme uses cellulose to bind while oxidizing neighboring xylan chains. No activity is observed with wheat arabinoxylan, birchwood glucuronoxylan and oat spelt xylan alone Thermothelomyces thermophilus C1 ?
-
 ?
[(1->4)-beta-D-glucosyl]n+m + AH2 + O2
-
 Thermothelomyces thermophilus [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?
[(1->4)-beta-D-glucosyl]n+m + AH2 + O2
-
 Thermothelomyces thermophilus C1 [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + A + H2O
-
 ?

Synonyms

Synonyms Comment Organism
LPMO ambiguous Thermothelomyces thermophilus
LPMO9A
-
 Thermothelomyces thermophilus
lytic polysaccharide monooxygenase ambiguous Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay temperature Thermothelomyces thermophilus