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Literature summary for 1.14.99.53 extracted from

  • Nakagawa, Y.S.; Kudo, M.; Loose, J.S.; Ishikawa, T.; Totani, K.; Eijsink, V.G.; Vaaje-Kolstad, G.
    A small lytic polysaccharide monooxygenase from Streptomyces griseus targeting alpha- and beta-chitin (2015), FEBS J., 282, 1065-1079.
    View publication on PubMed
Search for more literature for 1.14.99.53

Cloned(Commentary)

Cloned (Comment) Organism
expression using a Brevibacillus-based expression system Streptomyces griseus subsp. griseus

Protein Variants

Protein Variants Comment Organism
Y56W mutation shows no detectable effect on substrate-binding preferences but, in synergy experiments with chitinases, the mutant appears to be more efficient on alpha-chitin Streptomyces griseus subsp. griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus subsp. griseus B1VN59
-
-
Streptomyces griseus subsp. griseus JCM 4626 B1VN59
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + ascorbate + O2 enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin Streptomyces griseus subsp. griseus C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products show oxidation of the C1 carbon leading to aldonic acids ?
alpha-chitin + ascorbate + O2 enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin Streptomyces griseus subsp. griseus JCM 4626 C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products show oxidation of the C1 carbon leading to aldonic acids ?
beta-chitin + ascorbate + O2 enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin Streptomyces griseus subsp. griseus C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids ?
beta-chitin + ascorbate + O2 enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin Streptomyces griseus subsp. griseus JCM 4626 C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids ?

Synonyms

Synonyms Comment Organism
LPMO10F
-
 Streptomyces griseus subsp. griseus
SGR_6855
-
 Streptomyces griseus subsp. griseus

General Information

General Information Comment Organism
physiological function enzyme increases chitin solubilization yields of chitinases by up to 30fold and 20fold for alpha- and beta-chitin, respectively. The addition of LPMO10F leads to a substantial increase in the (GlcNAc)2:GlcNAc product ratio of chitinases, in reactions with alpha-chitin only Streptomyces griseus subsp. griseus