Application | Comment | Organism |
---|---|---|
analysis | development of a chitinase-coupled assay method and an assay based on consumption of ascorbate. There is no H2O2 production in the presence of colloidal chitin at 4 mg/ml | Bacillus atrophaeus |
Cloned (Comment) | Organism |
---|---|
expression in Baciluus subtilis | Bacillus atrophaeus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | enzyme activity is decreased by NaCl | Bacillus atrophaeus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus atrophaeus | A0A0H3E2X6 | - |
- |
Bacillus atrophaeus 1942 | A0A0H3E2X6 | - |
- |
Purification (Comment) | Organism |
---|---|
expression Bacillus subtilis yields a simple purification process without complicated periplasmic fractionation, as well as improved productivity | Bacillus atrophaeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin + ascorbate + O2 | - |
Bacillus atrophaeus | [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O | - |
? | |
colloidal chitin + ascorbate + O2 | - |
Bacillus atrophaeus 1942 | [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BATR1942_08650 | - |
Bacillus atrophaeus |
LPMO10 | - |
Bacillus atrophaeus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Tris/HCl buffer | Bacillus atrophaeus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
ammonium acetate buffer, 34% of maximum activity | Bacillus atrophaeus |
General Information | Comment | Organism |
---|---|---|
physiological function | LPMO10 and a chitinase mutually enhance each otherยs activities upon degrading chitin as the substrate | Bacillus atrophaeus |