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show all sequences of 1.14.99.52

Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway

Hu, W.; Song, H.; Sae Her, A.; Bak, D.W.; Naowarojna, N.; Elliott, S.J.; Qin, L.; Chen, X.; Liu, P.; Org. Lett. 16, 5382-5385 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering, but the kinetic properties and the presence of side-reaction render OvoA not suitable for use in ergothioneine production through metabolic engineering
Erwinia tasmaniensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
non-heme iron, required for catalysis
Erwinia tasmaniensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + L-cysteine + O2
Erwinia tasmaniensis
reaction of EC 1.14.99.51
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
L-histidine + L-cysteine + O2
Erwinia tasmaniensis
-
S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Erwinia tasmaniensis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + L-cysteine + O2
reaction of EC 1.14.99.51
743388
Erwinia tasmaniensis
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
L-histidine + L-cysteine + O2
-
743388
Erwinia tasmaniensis
S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
-
-
?
additional information
when OvoA's native substrate histidine is replaced by hercynine, OvoA can catalyze a one-step transformation reaction of hercynylcysteine S-oxide synthase, EC 1.14.99.51, resulting in kobs changes by 2fold for hercynine, while Km for cysteine increases from 0.3 mM to 3.19 mM. OvoA preferentially uses histidine and cysteine. OvoA also catalyzes the oxidation of cysteine to cysteine sulfinic acid, EC 1.13.11.20. Enzyme activity determination using : a 1H NMR assay of chemical shift of the imidazole hydrogen atoms
743388
Erwinia tasmaniensis
?
-
-
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering, but the kinetic properties and the presence of side-reaction render OvoA not suitable for use in ergothioneine production through metabolic engineering
Erwinia tasmaniensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
non-heme iron, required for catalysis
Erwinia tasmaniensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + L-cysteine + O2
Erwinia tasmaniensis
reaction of EC 1.14.99.51
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
L-histidine + L-cysteine + O2
Erwinia tasmaniensis
-
S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + L-cysteine + O2
reaction of EC 1.14.99.51
743388
Erwinia tasmaniensis
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
L-histidine + L-cysteine + O2
-
743388
Erwinia tasmaniensis
S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
-
-
?
additional information
when OvoA's native substrate histidine is replaced by hercynine, OvoA can catalyze a one-step transformation reaction of hercynylcysteine S-oxide synthase, EC 1.14.99.51, resulting in kobs changes by 2fold for hercynine, while Km for cysteine increases from 0.3 mM to 3.19 mM. OvoA preferentially uses histidine and cysteine. OvoA also catalyzes the oxidation of cysteine to cysteine sulfinic acid, EC 1.13.11.20. Enzyme activity determination using : a 1H NMR assay of chemical shift of the imidazole hydrogen atoms
743388
Erwinia tasmaniensis
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
OvoA and EgtB, EC 1.14.99.50, are related in sequence, while they are biochemically distinct
Erwinia tasmaniensis
metabolism
enzyme OvoA is responsible for the oxidative C-S bond formation in ovothiol biosynthesis. OvoA in ovothiol biosynthesis has a relaxed substrate specificity
Erwinia tasmaniensis
General Information (protein specific)
General Information
Commentary
Organism
evolution
OvoA and EgtB, EC 1.14.99.50, are related in sequence, while they are biochemically distinct
Erwinia tasmaniensis
metabolism
enzyme OvoA is responsible for the oxidative C-S bond formation in ovothiol biosynthesis. OvoA in ovothiol biosynthesis has a relaxed substrate specificity
Erwinia tasmaniensis
Other publictions for EC 1.14.99.52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Erwinia tasmaniensis
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2014
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745960
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Cysteine oxidation reactions ...
Erwinia tasmaniensis
Org. Lett.
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734024
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133
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