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show all sequences of 1.14.99.51

Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway

Hu, W.; Song, H.; Sae Her, A.; Bak, D.W.; Naowarojna, N.; Elliott, S.J.; Qin, L.; Chen, X.; Liu, P.; Org. Lett. 16, 5382-5385 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering
Neurospora crassa
Cloned(Commentary)
Commentary
Organism
gene egt1, Egt1 recombinant overexpression from vector pASK-IBA3+ in Escherichia coli
Neurospora crassa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.39
-
hercynine
pH and temperature not specified in the publication, with gamma-L-glutamyl-L-cysteine
Neurospora crassa
0.436
-
hercynine
pH and temperature not specified in the publication, with L-Cys
Neurospora crassa
0.603
-
L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
7.68
-
gamma-L-glutamyl-L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for catalysis, the enzyme contains about 0.90 Fe per Egt1 monomer
Neurospora crassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + gamma-L-glutamyl-L-cysteine + O2
Neurospora crassa
reaction of EC 1.14.99.50, low activity
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + gamma-L-glutamyl-L-cysteine + O2
Neurospora crassa ATCC 24698
reaction of EC 1.14.99.50, low activity
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + L-cysteine + O2
Neurospora crassa
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + L-cysteine + O2
Neurospora crassa ATCC 24698
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
Q7RX33
-
-
Neurospora crassa ATCC 24698
Q7RX33
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + gamma-L-glutamyl-L-cysteine + O2
reaction of EC 1.14.99.50, low activity
743388
Neurospora crassa
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + gamma-L-glutamyl-L-cysteine + O2
reaction of EC 1.14.99.50, low activity
743388
Neurospora crassa ATCC 24698
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
-
743388
Neurospora crassa
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
-
743388
Neurospora crassa ATCC 24698
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
additional information
three different assay methods for enzyme activity determination: a 1H NMR assay of chemical shift of the imidazole hydrogen atoms, an oxygen consumption assay, and a 13C NMR assay to monitor the presence of other cysteine related oxygen consumption. Egt1 from Neurospora crassa prefers cysteine as substrate compared to gamma-L-glutamyl-L-cysteine, Egt1 has a 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys
743388
Neurospora crassa
?
-
-
-
-
additional information
three different assay methods for enzyme activity determination: a 1H NMR assay of chemical shift of the imidazole hydrogen atoms, an oxygen consumption assay, and a 13C NMR assay to monitor the presence of other cysteine related oxygen consumption. Egt1 from Neurospora crassa prefers cysteine as substrate compared to gamma-L-glutamyl-L-cysteine, Egt1 has a 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys
743388
Neurospora crassa ATCC 24698
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.461
-
gamma-L-glutamyl-L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
0.461
-
hercynine
pH and temperature not specified in the publication, with gamma-L-glutamyl-L-cysteine
Neurospora crassa
2.267
-
hercynine
pH and temperature not specified in the publication, with L-Cys
Neurospora crassa
2.267
-
L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
Application (protein specific)
Application
Commentary
Organism
synthesis
the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering
Neurospora crassa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene egt1, Egt1 recombinant overexpression from vector pASK-IBA3+ in Escherichia coli
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.39
-
hercynine
pH and temperature not specified in the publication, with gamma-L-glutamyl-L-cysteine
Neurospora crassa
0.436
-
hercynine
pH and temperature not specified in the publication, with L-Cys
Neurospora crassa
0.603
-
L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
7.68
-
gamma-L-glutamyl-L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for catalysis, the enzyme contains about 0.90 Fe per Egt1 monomer
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + gamma-L-glutamyl-L-cysteine + O2
Neurospora crassa
reaction of EC 1.14.99.50, low activity
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + gamma-L-glutamyl-L-cysteine + O2
Neurospora crassa ATCC 24698
reaction of EC 1.14.99.50, low activity
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + L-cysteine + O2
Neurospora crassa
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + L-cysteine + O2
Neurospora crassa ATCC 24698
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + gamma-L-glutamyl-L-cysteine + O2
reaction of EC 1.14.99.50, low activity
743388
Neurospora crassa
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + gamma-L-glutamyl-L-cysteine + O2
reaction of EC 1.14.99.50, low activity
743388
Neurospora crassa ATCC 24698
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
-
743388
Neurospora crassa
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
-
743388
Neurospora crassa ATCC 24698
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
additional information
three different assay methods for enzyme activity determination: a 1H NMR assay of chemical shift of the imidazole hydrogen atoms, an oxygen consumption assay, and a 13C NMR assay to monitor the presence of other cysteine related oxygen consumption. Egt1 from Neurospora crassa prefers cysteine as substrate compared to gamma-L-glutamyl-L-cysteine, Egt1 has a 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys
743388
Neurospora crassa
?
-
-
-
-
additional information
three different assay methods for enzyme activity determination: a 1H NMR assay of chemical shift of the imidazole hydrogen atoms, an oxygen consumption assay, and a 13C NMR assay to monitor the presence of other cysteine related oxygen consumption. Egt1 from Neurospora crassa prefers cysteine as substrate compared to gamma-L-glutamyl-L-cysteine, Egt1 has a 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys
743388
Neurospora crassa ATCC 24698
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.461
-
gamma-L-glutamyl-L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
0.461
-
hercynine
pH and temperature not specified in the publication, with gamma-L-glutamyl-L-cysteine
Neurospora crassa
2.267
-
hercynine
pH and temperature not specified in the publication, with L-Cys
Neurospora crassa
2.267
-
L-cysteine
pH and temperature not specified in the publication
Neurospora crassa
General Information
General Information
Commentary
Organism
malfunction
Egt1 knockout in Neurospora crassa makes the mutant much more sensitive to oxidative stress compared to wild-type
Neurospora crassa
metabolism
the fungus Neurospora crassa has a concise ergothioneine biosynthetic pathway, its nonheme iron enzyme Egt1 makes use of cysteine instead of gamma-Glu-Cys (like EgtB, EC 1.14.99.50) as the substrate. Such a change of substrate preference eliminates the competition between ergothioneine and glutathione biosyntheses. In Neuropsora crassa ergothioneine biosynthesis, there are reactions distinct from the pathway via EgtB: the oxidative C-S bond formation and the subsequent C-S bond cleavage reaction, which result in a net transfer of the sulfur atom form cysteine to histidine imidazole side-chain
Neurospora crassa
General Information (protein specific)
General Information
Commentary
Organism
malfunction
Egt1 knockout in Neurospora crassa makes the mutant much more sensitive to oxidative stress compared to wild-type
Neurospora crassa
metabolism
the fungus Neurospora crassa has a concise ergothioneine biosynthetic pathway, its nonheme iron enzyme Egt1 makes use of cysteine instead of gamma-Glu-Cys (like EgtB, EC 1.14.99.50) as the substrate. Such a change of substrate preference eliminates the competition between ergothioneine and glutathione biosyntheses. In Neuropsora crassa ergothioneine biosynthesis, there are reactions distinct from the pathway via EgtB: the oxidative C-S bond formation and the subsequent C-S bond cleavage reaction, which result in a net transfer of the sulfur atom form cysteine to histidine imidazole side-chain
Neurospora crassa
Other publictions for EC 1.14.99.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732793
Pluskal
Genetic and metabolomic dissec ...
Schizosaccharomyces pombe
PLoS One
9
e97774
2014
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-
-
-
-
-
-
-
-
1
-
1
-
7
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
734829
Hu
Bioinformatic and biochemical ...
Neurospora crassa, Neurospora crassa ATCC 24698
Org. Lett.
16
5382-5385
2014
-
1
1
-
-
-
-
4
-
1
-
2
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
743388
Hu
Bioinformatic and biochemical ...
Neurospora crassa, Neurospora crassa ATCC 24698
Org. Lett.
16
5382-5385
2014
-
1
1
-
-
-
-
4
-
1
-
4
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
2
2
-
-
-
743662
Pluskal
Genetic and metabolomic disse ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972
PLoS ONE
9
e97774
2014
-
-
1
-
1
-
-
-
-
1
-
4
-
8
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
733921
Bello
The Neurospora crassa mutant N ...
Neurospora crassa, Neurospora crassa ATCC 24698, no activity in Bremia lactucae, no activity in Candida albicans, no activity in Pichia ssp., no activity in Saccharomyces cerevisiae
Fungal Genet. Biol.
49
160-172
2012
-
-
-
-
1
-
-
-
-
-
-
2
-
8
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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2
-
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-
-
-
2
-
-
2
-
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-
-
-
-
-
-
4
4
-
-
-