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show all sequences of 1.14.99.51

The Neurospora crassa mutant NcDELTAEgt-1 identifies an ergothioneine biosynthetic gene and demonstrates that ergothioneine enhances conidial survival and protects against peroxide toxicity during conidial germination

Bello, M.; Barrera-Perez, V.; Morin, D.; Epstein, L.; Fungal Genet. Biol. 49, 160-172 (2012)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
the NcDELTAEgt-1 enzyme knockout strain does not produce ergothioneine. The mutant strain is not pleiotropically affected in rate of hyphal elongation in Vogel's medium either with or without ammonium nitrate and in the rate of germination of macroconidia on Vogel's medium, the mutant is also not differently affected from the wild-type by menadione and cupric sulfate, but germination of NcDELTAEgt-1 conidia is significantly more sensitive to tert-butyl hydroperoxide than the wild-type, phenotype, overview
Neurospora crassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + L-cysteine + O2
Neurospora crassa
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + L-cysteine + O2
Neurospora crassa ATCC 24698
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
Q7RX33
gene egt1 or NCU04343
-
Neurospora crassa ATCC 24698
Q7RX33
gene egt1 or NCU04343
-
no activity in Bremia lactucae
-
-
-
no activity in Candida albicans
-
-
-
no activity in Pichia ssp.
-
-
-
no activity in Saccharomyces cerevisiae
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
conidium
-
Neurospora crassa
-
mycelium
-
Neurospora crassa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + L-cysteine + O2
-
733921
Neurospora crassa
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
-
733921
Neurospora crassa ATCC 24698
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the NcDELTAEgt-1 enzyme knockout strain does not produce ergothioneine. The mutant strain is not pleiotropically affected in rate of hyphal elongation in Vogel's medium either with or without ammonium nitrate and in the rate of germination of macroconidia on Vogel's medium, the mutant is also not differently affected from the wild-type by menadione and cupric sulfate, but germination of NcDELTAEgt-1 conidia is significantly more sensitive to tert-butyl hydroperoxide than the wild-type, phenotype, overview
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + L-cysteine + O2
Neurospora crassa
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
hercynine + L-cysteine + O2
Neurospora crassa ATCC 24698
-
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
conidium
-
Neurospora crassa
-
mycelium
-
Neurospora crassa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + L-cysteine + O2
-
733921
Neurospora crassa
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
-
733921
Neurospora crassa ATCC 24698
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
distribution of NcEgt-1 homologues in fungi, overview. The enzyme homologues are present in all of the true fungal groups in which ergothioneine is identified: Mucoromycotina (i.e., Mucorales), Agaricomycotina (i.e., Agaricales, Boletales, and Cantharellales), Pucciniomycotina (i.e., Sporidiobolales), and the Pezizomycotina (i.e., Eurotiales, Glomerellales, Hypocreales, and Sordariales)
Neurospora crassa
malfunction
no ergothioneine is formed in the egt1-knockout mutant strain of Neurospora crassa, the mutant conidia show higher sensitivity to tert-butyl hydroperoxide compared to the wild-type strain, phenotype, overview
Neurospora crassa
metabolism
the enzyme catalyzes a step in the ergothioneine, EGT, biosynthetic pathway
Neurospora crassa
physiological function
the enzyme is essentially involved in ergothioneine biosynthesis, which enhances conidial survival and protects against peroxide toxicity during conidial germination, overview. Ergothioneine does not have discernible pleiotropic affects and does not appear to protect against either Cu2+ toxicity or superoxide in vivo
Neurospora crassa
General Information (protein specific)
General Information
Commentary
Organism
evolution
distribution of NcEgt-1 homologues in fungi, overview. The enzyme homologues are present in all of the true fungal groups in which ergothioneine is identified: Mucoromycotina (i.e., Mucorales), Agaricomycotina (i.e., Agaricales, Boletales, and Cantharellales), Pucciniomycotina (i.e., Sporidiobolales), and the Pezizomycotina (i.e., Eurotiales, Glomerellales, Hypocreales, and Sordariales)
Neurospora crassa
malfunction
no ergothioneine is formed in the egt1-knockout mutant strain of Neurospora crassa, the mutant conidia show higher sensitivity to tert-butyl hydroperoxide compared to the wild-type strain, phenotype, overview
Neurospora crassa
metabolism
the enzyme catalyzes a step in the ergothioneine, EGT, biosynthetic pathway
Neurospora crassa
physiological function
the enzyme is essentially involved in ergothioneine biosynthesis, which enhances conidial survival and protects against peroxide toxicity during conidial germination, overview. Ergothioneine does not have discernible pleiotropic affects and does not appear to protect against either Cu2+ toxicity or superoxide in vivo
Neurospora crassa
Other publictions for EC 1.14.99.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732793
Pluskal
Genetic and metabolomic dissec ...
Schizosaccharomyces pombe
PLoS One
9
e97774
2014
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-
-
-
-
-
-
-
-
1
-
1
-
7
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
734829
Hu
Bioinformatic and biochemical ...
Neurospora crassa, Neurospora crassa ATCC 24698
Org. Lett.
16
5382-5385
2014
-
1
1
-
-
-
-
4
-
1
-
2
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
743388
Hu
Bioinformatic and biochemical ...
Neurospora crassa, Neurospora crassa ATCC 24698
Org. Lett.
16
5382-5385
2014
-
1
1
-
-
-
-
4
-
1
-
4
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
2
2
-
-
-
743662
Pluskal
Genetic and metabolomic disse ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972
PLoS ONE
9
e97774
2014
-
-
1
-
1
-
-
-
-
1
-
4
-
8
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
733921
Bello
The Neurospora crassa mutant N ...
Neurospora crassa, Neurospora crassa ATCC 24698, no activity in Bremia lactucae, no activity in Candida albicans, no activity in Pichia ssp., no activity in Saccharomyces cerevisiae
Fungal Genet. Biol.
49
160-172
2012
-
-
-
-
1
-
-
-
-
-
-
2
-
8
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
4
4
-
-
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