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show all sequences of 1.14.99.51

Genetic and metabolomic dissection of the ergothioneine and selenoneine biosynthetic pathway in the fission yeast, S. pombe, and construction of an overproduction system

Pluskal, T.; Ueno, M.; Yanagida, M.; PLoS One 9, e97774 (2014)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
the enzyme requires Fe2+ for activity
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + L-cysteine + O2
Schizosaccharomyces pombe
the enzyme is part of the biosynthesis pathway of ergothioneine
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Schizosaccharomyces pombe
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + L-cysteine + O2
the enzyme is part of the biosynthesis pathway of ergothioneine
732793
Schizosaccharomyces pombe
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
hercynine i.e. Nalpha,Nalpha,Nalpha-trimethyl-L-histidine
732793
Schizosaccharomyces pombe
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-selenocysteine + O2
hercynine i.e. Nalpha,Nalpha,Nalpha-trimethyl-L-histidine
732793
Schizosaccharomyces pombe
S-(hercyn-2-yl)-L-selenocysteine S-oxide + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the enzyme, found in fungal species, is part of a fusion protein that also has the the activity of EC 2.1.1.44, L-histidine Nalpha-methyltransferase
Schizosaccharomyces pombe
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
the enzyme requires Fe2+ for activity
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hercynine + L-cysteine + O2
Schizosaccharomyces pombe
the enzyme is part of the biosynthesis pathway of ergothioneine
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hercynine + L-cysteine + O2
the enzyme is part of the biosynthesis pathway of ergothioneine
732793
Schizosaccharomyces pombe
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-cysteine + O2
hercynine i.e. Nalpha,Nalpha,Nalpha-trimethyl-L-histidine
732793
Schizosaccharomyces pombe
S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
-
-
?
hercynine + L-selenocysteine + O2
hercynine i.e. Nalpha,Nalpha,Nalpha-trimethyl-L-histidine
732793
Schizosaccharomyces pombe
S-(hercyn-2-yl)-L-selenocysteine S-oxide + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the enzyme, found in fungal species, is part of a fusion protein that also has the the activity of EC 2.1.1.44, L-histidine Nalpha-methyltransferase
Schizosaccharomyces pombe
General Information
General Information
Commentary
Organism
malfunction
the DELTA egt1 deletion mutant shows a complete absence of ergothioneine and all its precursors
Schizosaccharomyces pombe
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the DELTA egt1 deletion mutant shows a complete absence of ergothioneine and all its precursors
Schizosaccharomyces pombe
Other publictions for EC 1.14.99.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732793
Pluskal
Genetic and metabolomic dissec ...
Schizosaccharomyces pombe
PLoS One
9
e97774
2014
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1
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1
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7
-
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-
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3
1
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-
1
-
1
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-
-
-
-
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3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
734829
Hu
Bioinformatic and biochemical ...
Neurospora crassa, Neurospora crassa ATCC 24698
Org. Lett.
16
5382-5385
2014
-
1
1
-
-
-
-
4
-
1
-
2
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
743388
Hu
Bioinformatic and biochemical ...
Neurospora crassa, Neurospora crassa ATCC 24698
Org. Lett.
16
5382-5385
2014
-
1
1
-
-
-
-
4
-
1
-
4
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
2
2
-
-
-
743662
Pluskal
Genetic and metabolomic disse ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972
PLoS ONE
9
e97774
2014
-
-
1
-
1
-
-
-
-
1
-
4
-
8
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
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-
1
-
4
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
733921
Bello
The Neurospora crassa mutant N ...
Neurospora crassa, Neurospora crassa ATCC 24698, no activity in Bremia lactucae, no activity in Candida albicans, no activity in Pichia ssp., no activity in Saccharomyces cerevisiae
Fungal Genet. Biol.
49
160-172
2012
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1
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2
-
8
-
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-
-
2
-
-
2
-
-
-
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-
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1
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2
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-
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2
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2
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4
4
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