BRENDA - Enzyme Database
show all sequences of 1.14.99.40

Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species

Collins, H.F.; Biedendieck, R.; Leech, H.K.; Gray, M.; Escalante-Semerena, J.C.; McLean, K.J.; Munro, A.W.; Rigby, S.E.; Warren, M.J.; Lawrence, A.D.; PLoS ONE 8, e55708 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene bluB, expression in Escherichia coli strain BL21star(DE3)pLysS
Bacillus megaterium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
FMNH2 + NADH + H+ + O2
Bacillus megaterium
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
?
FMNH2 + NADH + H+ + O2
Bacillus megaterium DSM 319
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bacillus megaterium
D5DCL0
gene bluB
-
Bacillus megaterium DSM 319
D5DCL0
gene bluB
-
Reaction
Reaction
Commentary
Organism
Reaction ID
FMNH2 + NADH + H+ + O2 = 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + other product
first step in the proposed mechanism is the activation of molecular oxygen forming a 4a-peroxyflavin intermediate, the 4a-peroxyflavin is generated rapidly and is followed by a much slower decay phase, mechanism overview
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FMNH2 + NADH + H+ + O2
-
728590
Bacillus megaterium
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
FMNH2 + NADH + H+ + O2
NADH is required to reduce FMN to FMNH2 which is the substrate for the BluB reaction. BluB forms a peroxyflavin intermediate
728590
Bacillus megaterium
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
FMNH2 + NADH + H+ + O2
-
728590
Bacillus megaterium DSM 319
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
FMNH2 + NADH + H+ + O2
NADH is required to reduce FMN to FMNH2 which is the substrate for the BluB reaction. BluB forms a peroxyflavin intermediate
728590
Bacillus megaterium DSM 319
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BluB
-
Bacillus megaterium
dimethylbenzimidazole synthase
-
Bacillus megaterium
DMB-synthase
-
Bacillus megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus megaterium
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is required to reduce FMN to FMNH2 which is the substrate for the BluB reaction
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bluB, expression in Escherichia coli strain BL21star(DE3)pLysS
Bacillus megaterium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is required to reduce FMN to FMNH2 which is the substrate for the BluB reaction
Bacillus megaterium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
FMNH2 + NADH + H+ + O2
Bacillus megaterium
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
?
FMNH2 + NADH + H+ + O2
Bacillus megaterium DSM 319
-
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FMNH2 + NADH + H+ + O2
-
728590
Bacillus megaterium
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
FMNH2 + NADH + H+ + O2
NADH is required to reduce FMN to FMNH2 which is the substrate for the BluB reaction. BluB forms a peroxyflavin intermediate
728590
Bacillus megaterium
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
FMNH2 + NADH + H+ + O2
-
728590
Bacillus megaterium DSM 319
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
FMNH2 + NADH + H+ + O2
NADH is required to reduce FMN to FMNH2 which is the substrate for the BluB reaction. BluB forms a peroxyflavin intermediate
728590
Bacillus megaterium DSM 319
5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD+ + ?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus megaterium
General Information
General Information
Commentary
Organism
additional information
CbiY from Bacillus megaterium is not a BluB protein
Bacillus megaterium
General Information (protein specific)
General Information
Commentary
Organism
additional information
CbiY from Bacillus megaterium is not a BluB protein
Bacillus megaterium
Other publictions for EC 1.14.99.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728590
Collins
Bacillus megaterium has both a ...
Bacillus megaterium, Bacillus megaterium DSM 319
PLoS ONE
8
e55708
2013
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1
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2
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2
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1
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4
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3
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1
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1
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1
1
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4
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1
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1
1
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728760
Yu
Active site residues critical ...
Sinorhizobium meliloti
Protein Sci.
21
839-849
2012
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1
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15
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1
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1
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1
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2
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3
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1
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1
1
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15
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1
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1
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2
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1
1
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