BRENDA - Enzyme Database show
show all sequences of 1.14.99.4

cDNA cloning, heterologous expression, and characterization of mouse CYP2G1, an olfactory-specific steroid hydroxylase

Hua, Z.; Zhang, Q.; Su, T.; Lipinskas, T.W.; Ding, X.; Arch. Biochem. Biophys. 340, 208-214 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cDNA obtained by PCR, cloning into a baculoviral expression vector pVL1392, production of the enzyme in culturd insect cells and heterologous expression of CYP2G1 in rabbits and rats
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0051
-
testosterone
-
Mus musculus
0.0076
-
progesterone
-
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Mus musculus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Progesterone + NADPH + O2
Mus musculus
-
Testosterone acetate + NADP+ + H2O
-
Mus musculus
?
Progesterone + NADPH + O2
Rattus norvegicus
-
Testosterone acetate + NADP+ + H2O
-
Rattus norvegicus
?
Progesterone + NADPH + O2
Oryctolagus cuniculus
-
Testosterone acetate + NADP+ + H2O
-
Oryctolagus cuniculus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
mouse, strain C57BL/6
-
Oryctolagus cuniculus
-
rabbit
-
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
olfactory mucosa
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5alpha-dihydrotestosterone + NADPH + O2
-
438221
Oryctolagus cuniculus
?
-
-
-
?
androstenedione + NADPH + O2
-
438221
Oryctolagus cuniculus
?
-
-
-
?
estradiol + NADPH + O2
-
438221
Oryctolagus cuniculus
?
-
-
-
?
Progesterone + NADPH + O2
-
438221
Mus musculus
Testosterone acetate + NADP+ + H2O
-
438221
Mus musculus
?
Progesterone + NADPH + O2
-
438221
Rattus norvegicus
Testosterone acetate + NADP+ + H2O
-
438221
Rattus norvegicus
?
Progesterone + NADPH + O2
-
438221
Oryctolagus cuniculus
Testosterone acetate + NADP+ + H2O
-
438221
Oryctolagus cuniculus
?
testosterone + NADPH + O2
-
438221
Oryctolagus cuniculus
6beta-hydroxytestosterone + NADP+ + H2O
-
438221
Oryctolagus cuniculus
?
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNA obtained by PCR, cloning into a baculoviral expression vector pVL1392, production of the enzyme in culturd insect cells and heterologous expression of CYP2G1 in rabbits and rats
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0051
-
testosterone
-
Mus musculus
0.0076
-
progesterone
-
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Mus musculus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Progesterone + NADPH + O2
Mus musculus
-
Testosterone acetate + NADP+ + H2O
-
Mus musculus
?
Progesterone + NADPH + O2
Rattus norvegicus
-
Testosterone acetate + NADP+ + H2O
-
Rattus norvegicus
?
Progesterone + NADPH + O2
Oryctolagus cuniculus
-
Testosterone acetate + NADP+ + H2O
-
Oryctolagus cuniculus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
olfactory mucosa
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5alpha-dihydrotestosterone + NADPH + O2
-
438221
Oryctolagus cuniculus
?
-
-
-
?
androstenedione + NADPH + O2
-
438221
Oryctolagus cuniculus
?
-
-
-
?
estradiol + NADPH + O2
-
438221
Oryctolagus cuniculus
?
-
-
-
?
Progesterone + NADPH + O2
-
438221
Mus musculus
Testosterone acetate + NADP+ + H2O
-
438221
Mus musculus
?
Progesterone + NADPH + O2
-
438221
Rattus norvegicus
Testosterone acetate + NADP+ + H2O
-
438221
Rattus norvegicus
?
Progesterone + NADPH + O2
-
438221
Oryctolagus cuniculus
Testosterone acetate + NADP+ + H2O
-
438221
Oryctolagus cuniculus
?
testosterone + NADPH + O2
-
438221
Oryctolagus cuniculus
6beta-hydroxytestosterone + NADP+ + H2O
-
438221
Oryctolagus cuniculus
?
Other publictions for EC 1.14.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715071
Longo
Cisplatin induced toxicity in ...
Rattus norvegicus, Rattus norvegicus Wistar
Food Chem. Toxicol.
49
233-237
2011
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1
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-
166
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2
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2
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1
1
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-
659812
Lenasi
Specific interactions of stero ...
Rhizopus stolonifer
J. Steroid Biochem. Mol. Biol.
91
273-284
2004
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1
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438220
Berrie
Purification and characterizat ...
Streptomyces roseochromogenes
Biochem. Soc. Trans.
25
18S
1997
1
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1
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1
1
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2
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1
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2
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1
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1
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1
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1
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1
1
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1
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2
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-
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438221
Hua
cDNA cloning, heterologous exp ...
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Arch. Biochem. Biophys.
340
208-214
1997
-
-
1
-
-
-
-
2
1
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-
3
-
7
-
-
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-
4
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7
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1
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2
1
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3
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4
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7
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-
438219
Straub
Differential effects of mutati ...
Chlorocebus aethiops
Biochemistry
33
8029-8034
1994
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-
-
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-
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1
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2
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1
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1
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1
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-
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438217
Schlenk
Channel catfish liver monooxyg ...
Ictalurus punctatus, Oncorhynchus mykiss, Oryctolagus cuniculus, Sus scrofa
Biochem. Pharmacol.
45
217-221
1993
-
2
-
-
-
-
-
-
1
-
-
4
-
4
-
-
1
-
-
4
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
-
-
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-
1
-
-
4
-
-
-
1
-
4
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
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-
438218
Straub
Hydrophobic side chain require ...
Chlorocebus aethiops
Arch. Biochem. Biophys.
306
521-527
1993
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
-
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-
438216
Rahim
Mechanisms of steroid oxidatio ...
Amorphotheca resinae, Ilyonectria destructans, Ilyonectria destructans ACC 11011
J. Biol. Chem.
241
3615-3623
1966
-
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4
4
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3
-
3
-
-
1
-
-
-
-
2
13
-
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-
-
-
1
-
2
1
-
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-
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-
1
-
-
4
-
4
-
-
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-
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3
-
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-
1
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-
2
13
-
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1
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2
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