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show all sequences of 1.14.99.4

Purification and characterization of progesterone hydroxylase, cytochrome P-450 from Streptomyces roseochromogenes ATCC 13400

Berrie, J.R.; Williams, R.A.D.; Smith, K.E.; Biochem. Soc. Trans. 25, 18S (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
coumarin
hydroxylase activity is amplifiable by coumarin, increases 50% above that found constitutively
Streptomyces roseochromogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
ketoconazole
-
Streptomyces roseochromogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
PAGE, gel filtration
Streptomyces roseochromogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Progesterone + NADPH + O2
Streptomyces roseochromogenes
-
Testosterone acetate + NADP+ + H2O
-
Streptomyces roseochromogenes
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces roseochromogenes
-
ATCC 13400
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces roseochromogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Progesterone + NADPH + O2
-
438220
Streptomyces roseochromogenes
Testosterone acetate + NADP+ + H2O
-
438220
Streptomyces roseochromogenes
?
progesterone + NADPH + O2
-
438220
Streptomyces roseochromogenes
16alpha-hydroxyprogesterone + 2beta,16alpha-dihydroxyprogesterone + NADP+ + H2O
-
438220
Streptomyces roseochromogenes
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Streptomyces roseochromogenes
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
coumarin
hydroxylase activity is amplifiable by coumarin, increases 50% above that found constitutively
Streptomyces roseochromogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Streptomyces roseochromogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ketoconazole
-
Streptomyces roseochromogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
PAGE, gel filtration
Streptomyces roseochromogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Progesterone + NADPH + O2
Streptomyces roseochromogenes
-
Testosterone acetate + NADP+ + H2O
-
Streptomyces roseochromogenes
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces roseochromogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Progesterone + NADPH + O2
-
438220
Streptomyces roseochromogenes
Testosterone acetate + NADP+ + H2O
-
438220
Streptomyces roseochromogenes
?
progesterone + NADPH + O2
-
438220
Streptomyces roseochromogenes
16alpha-hydroxyprogesterone + 2beta,16alpha-dihydroxyprogesterone + NADP+ + H2O
-
438220
Streptomyces roseochromogenes
?
Other publictions for EC 1.14.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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438220
Berrie
Purification and characterizat ...
Streptomyces roseochromogenes
Biochem. Soc. Trans.
25
18S
1997
1
-
-
-
-
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1
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1
1
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2
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1
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2
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1
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1
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1
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1
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1
1
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1
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2
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-
-
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438221
Hua
cDNA cloning, heterologous exp ...
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Arch. Biochem. Biophys.
340
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1
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2
1
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3
-
7
-
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4
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7
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1
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2
1
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3
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4
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7
-
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-
438219
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Differential effects of mutati ...
Chlorocebus aethiops
Biochemistry
33
8029-8034
1994
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-
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-
-
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1
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2
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1
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1
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1
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438217
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Biochem. Pharmacol.
45
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1993
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2
-
-
-
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-
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1
-
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4
-
4
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1
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4
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6
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2
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2
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2
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1
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4
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1
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4
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6
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438218
Straub
Hydrophobic side chain require ...
Chlorocebus aethiops
Arch. Biochem. Biophys.
306
521-527
1993
-
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1
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1
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1
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1
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1
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438216
Rahim
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J. Biol. Chem.
241
3615-3623
1966
-
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4
4
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3
-
3
-
-
1
-
-
-
-
2
13
-
-
-
-
-
1
-
2
1
-
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-
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-
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1
-
-
4
-
4
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-
-
3
-
-
-
1
-
-
-
2
13
-
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1
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2
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