BRENDA - Enzyme Database show
show all sequences of 1.14.99.4

Mechanisms of steroid oxidation by microorganisms. XI. Enzymatic cleavage of the pregnane side chain

Rahim, M.A.; Sih, C.J.; J. Biol. Chem. 241, 3615-3623 (1966)

Data extracted from this reference:

General Stability
General Stability
Organism
1 mg/ml albumin stabilizes crude enzyme extract for 1 month when kept frozen
Ilyonectria destructans
10 h dialysis of purified enzyme against 0.01 M Tris buffer, pH 7.5, 0.005 M EDTA, leads to 50% loss of activity
Ilyonectria destructans
EDTA affords some protection, especially for crude extracts
Ilyonectria destructans
preparation is highly unstable
Ilyonectria destructans
Inhibitors
Inhibitors
Commentary
Organism
Structure
KCN
-
Ilyonectria destructans
methylene blue
-
Ilyonectria destructans
p-hydroxymercuribenzoate
-
Ilyonectria destructans
phenazine methosulfate
-
Ilyonectria destructans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Progesterone + NADPH + O2
Amorphotheca resinae
-
Testosterone acetate + NADP+ + H2O
-
Amorphotheca resinae
?
Progesterone + NADPH + O2
Ilyonectria destructans
-
Testosterone acetate + NADP+ + H2O
-
Ilyonectria destructans
?
Progesterone + NADPH + O2
Ilyonectria destructans ACC 11011
-
Testosterone acetate + NADP+ + H2O
-
Ilyonectria destructans ACC 11011
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Amorphotheca resinae
-
-
-
Ilyonectria destructans
-
ACC 11011
-
Ilyonectria destructans ACC 11011
-
ACC 11011
-
Purification (Commentary)
Commentary
Organism
-
Ilyonectria destructans
Storage Stability
Storage Stability
Organism
0-6°, 85% of the enzyme activity is lost in 24 h
Ilyonectria destructans
0°C, 80% enzyme activity is lost in 7 days, half-life of a highly purified preparation is 12 h
Ilyonectria destructans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains
438216
Ilyonectria destructans
16alpha-hydroxyandrost-4-ene-3,17-dione
-
438216
Ilyonectria destructans
?
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains
438216
Ilyonectria destructans ACC 11011
16alpha-hydroxyandrost-4-ene-3,17-dione
-
438216
Ilyonectria destructans ACC 11011
?
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains, about 210% the rate of the reaction with progesterone
438216
Ilyonectria destructans
androst-4-ene-3,17-dione + NADP+ + ?
-
438216
Ilyonectria destructans
?
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains, about 210% the rate of the reaction with progesterone
438216
Ilyonectria destructans ACC 11011
androst-4-ene-3,17-dione + NADP+ + ?
-
438216
Ilyonectria destructans ACC 11011
?
17alpha-methylpregn-4-ene-3,20-dione + NADPH + O2
about 4.3% the rate of the reaction with progesterone
438216
Ilyonectria destructans
?
-
-
-
?
deoxycorticosterone + NADPH + O2
degradation of pregnane side chains, about 64% the rate of the reaction with progesterone
438216
Ilyonectria destructans
testosterone + NADP+ + ?
-
438216
Ilyonectria destructans
?
deoxycorticosterone + NADPH + O2
degradation of pregnane side chains, about 64% the rate of the reaction with progesterone
438216
Ilyonectria destructans ACC 11011
testosterone + NADP+ + ?
-
438216
Ilyonectria destructans ACC 11011
?
additional information
cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates
438216
Ilyonectria destructans
?
-
-
-
-
additional information
cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates
438216
Ilyonectria destructans ACC 11011
?
-
-
-
-
pregna-4,16-diene-3,20-dione + NADPH + O2
degradation of pregnane side chains, about 104% the rate of the reaction with progesterone
438216
Ilyonectria destructans
androst-4-ene-3,17-dione
-
438216
Ilyonectria destructans
?
Progesterone + NADPH + O2
-
438216
Amorphotheca resinae
Testosterone acetate + NADP+ + H2O
-
438216
Amorphotheca resinae
?
Progesterone + NADPH + O2
-
438216
Ilyonectria destructans
Testosterone acetate + NADP+ + H2O
-
438216
Ilyonectria destructans
?
Progesterone + NADPH + O2
-
438216
Ilyonectria destructans ACC 11011
Testosterone acetate + NADP+ + H2O
-
438216
Ilyonectria destructans ACC 11011
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
progesterone, deoxycorticosterone, 17alpha-hydroxypregn-4-ene-3,20-dione as substrates
Ilyonectria destructans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.4
9
irreversible inactivated below
Ilyonectria destructans
7
8
most stable
Ilyonectria destructans
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
absolute requirement, FMN, FAD, NADH or 2-amino-6,7-dimethyl-4-hydroxy-5,6,7,8-tetrahydropteridine cannot replace NADPH as hydrogen-donor
Ilyonectria destructans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
absolute requirement, FMN, FAD, NADH or 2-amino-6,7-dimethyl-4-hydroxy-5,6,7,8-tetrahydropteridine cannot replace NADPH as hydrogen-donor
Ilyonectria destructans
General Stability (protein specific)
General Stability
Organism
1 mg/ml albumin stabilizes crude enzyme extract for 1 month when kept frozen
Ilyonectria destructans
10 h dialysis of purified enzyme against 0.01 M Tris buffer, pH 7.5, 0.005 M EDTA, leads to 50% loss of activity
Ilyonectria destructans
EDTA affords some protection, especially for crude extracts
Ilyonectria destructans
preparation is highly unstable
Ilyonectria destructans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCN
-
Ilyonectria destructans
methylene blue
-
Ilyonectria destructans
p-hydroxymercuribenzoate
-
Ilyonectria destructans
phenazine methosulfate
-
Ilyonectria destructans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Progesterone + NADPH + O2
Amorphotheca resinae
-
Testosterone acetate + NADP+ + H2O
-
Amorphotheca resinae
?
Progesterone + NADPH + O2
Ilyonectria destructans
-
Testosterone acetate + NADP+ + H2O
-
Ilyonectria destructans
?
Progesterone + NADPH + O2
Ilyonectria destructans ACC 11011
-
Testosterone acetate + NADP+ + H2O
-
Ilyonectria destructans ACC 11011
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ilyonectria destructans
Storage Stability (protein specific)
Storage Stability
Organism
0-6°, 85% of the enzyme activity is lost in 24 h
Ilyonectria destructans
0°C, 80% enzyme activity is lost in 7 days, half-life of a highly purified preparation is 12 h
Ilyonectria destructans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains
438216
Ilyonectria destructans
16alpha-hydroxyandrost-4-ene-3,17-dione
-
438216
Ilyonectria destructans
?
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains
438216
Ilyonectria destructans ACC 11011
16alpha-hydroxyandrost-4-ene-3,17-dione
-
438216
Ilyonectria destructans ACC 11011
?
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains, about 210% the rate of the reaction with progesterone
438216
Ilyonectria destructans
androst-4-ene-3,17-dione + NADP+ + ?
-
438216
Ilyonectria destructans
?
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2
degradation of pregnane side chains, about 210% the rate of the reaction with progesterone
438216
Ilyonectria destructans ACC 11011
androst-4-ene-3,17-dione + NADP+ + ?
-
438216
Ilyonectria destructans ACC 11011
?
17alpha-methylpregn-4-ene-3,20-dione + NADPH + O2
about 4.3% the rate of the reaction with progesterone
438216
Ilyonectria destructans
?
-
-
-
?
deoxycorticosterone + NADPH + O2
degradation of pregnane side chains, about 64% the rate of the reaction with progesterone
438216
Ilyonectria destructans
testosterone + NADP+ + ?
-
438216
Ilyonectria destructans
?
deoxycorticosterone + NADPH + O2
degradation of pregnane side chains, about 64% the rate of the reaction with progesterone
438216
Ilyonectria destructans ACC 11011
testosterone + NADP+ + ?
-
438216
Ilyonectria destructans ACC 11011
?
additional information
cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates
438216
Ilyonectria destructans
?
-
-
-
-
additional information
cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates
438216
Ilyonectria destructans ACC 11011
?
-
-
-
-
pregna-4,16-diene-3,20-dione + NADPH + O2
degradation of pregnane side chains, about 104% the rate of the reaction with progesterone
438216
Ilyonectria destructans
androst-4-ene-3,17-dione
-
438216
Ilyonectria destructans
?
Progesterone + NADPH + O2
-
438216
Amorphotheca resinae
Testosterone acetate + NADP+ + H2O
-
438216
Amorphotheca resinae
?
Progesterone + NADPH + O2
-
438216
Ilyonectria destructans
Testosterone acetate + NADP+ + H2O
-
438216
Ilyonectria destructans
?
Progesterone + NADPH + O2
-
438216
Ilyonectria destructans ACC 11011
Testosterone acetate + NADP+ + H2O
-
438216
Ilyonectria destructans ACC 11011
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
progesterone, deoxycorticosterone, 17alpha-hydroxypregn-4-ene-3,20-dione as substrates
Ilyonectria destructans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.4
9
irreversible inactivated below
Ilyonectria destructans
7
8
most stable
Ilyonectria destructans
Other publictions for EC 1.14.99.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715071
Longo
Cisplatin induced toxicity in ...
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Food Chem. Toxicol.
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1
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166
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1
1
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659812
Lenasi
Specific interactions of stero ...
Rhizopus stolonifer
J. Steroid Biochem. Mol. Biol.
91
273-284
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1
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438220
Berrie
Purification and characterizat ...
Streptomyces roseochromogenes
Biochem. Soc. Trans.
25
18S
1997
1
-
-
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1
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1
1
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2
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1
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1
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438221
Hua
cDNA cloning, heterologous exp ...
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Arch. Biochem. Biophys.
340
208-214
1997
-
-
1
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2
1
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3
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7
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4
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7
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1
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2
1
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3
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4
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7
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438219
Straub
Differential effects of mutati ...
Chlorocebus aethiops
Biochemistry
33
8029-8034
1994
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1
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2
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1
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1
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-
438217
Schlenk
Channel catfish liver monooxyg ...
Ictalurus punctatus, Oncorhynchus mykiss, Oryctolagus cuniculus, Sus scrofa
Biochem. Pharmacol.
45
217-221
1993
-
2
-
-
-
-
-
-
1
-
-
4
-
4
-
-
1
-
-
4
-
-
6
-
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-
-
-
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-
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2
-
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2
-
2
-
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1
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4
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1
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4
-
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6
-
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-
-
-
-
-
-
-
-
-
-
-
-
438218
Straub
Hydrophobic side chain require ...
Chlorocebus aethiops
Arch. Biochem. Biophys.
306
521-527
1993
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-
-
-
-
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-
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1
-
1
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1
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1
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1
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-
438216
Rahim
Mechanisms of steroid oxidatio ...
Amorphotheca resinae, Ilyonectria destructans, Ilyonectria destructans ACC 11011
J. Biol. Chem.
241
3615-3623
1966
-
-
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-
-
4
4
-
-
-
-
3
-
3
-
-
1
-
-
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2
13
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1
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2
1
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1
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4
-
4
-
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-
-
3
-
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-
1
-
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-
2
13
-
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1
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2
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