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Literature summary for 1.14.99.39 extracted from

  • Zhang, S.; Li, W.; Zhang, D.; Huang, X.; Qin, W.; Gu, J.
    Purification and characterization of a low-temperature ammonia monooxygenase from heterotrophic nitrifier Acinetobacter sp. Y16 (2015), Desalin. Water Treatment, 53, 257-262 .
No PubMed abstract available
Search for more literature for 1.14.99.39

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Acinetobacter sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ CuCl2 stimulates AMO activity in vitro Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NH3 + a reduced acceptor + O2 Acinetobacter sp.
-
 NH2OH + an acceptor + H2O
-
 ?
NH3 + a reduced acceptor + O2 Acinetobacter sp. Y16
-
 NH2OH + an acceptor + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
 isolated from Songhua River in winter, China
-
Acinetobacter sp. Y16
-
 isolated from Songhua River in winter, China
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 9.7fold by by anion-exchange chromatography and gel filtration as membrane-bound monomer Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.2
-
 purified native enzyme, pH 7.5, 15°C Acinetobacter sp.

Storage Stability

Storage Stability Organism
4-15°C, the purified enzyme activity is stable when stored at 4°C for five days, at 10°C for two days, and at 15°C for one day Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes the oxidation of ammonium without stabilizing agents in vitro at low temperature Acinetobacter sp. ?
-
 ?
additional information the enzyme catalyzes the oxidation of ammonium without stabilizing agents in vitro at low temperature Acinetobacter sp. Y16 ?
-
 ?
NH3 + a reduced acceptor + O2
-
 Acinetobacter sp. NH2OH + an acceptor + H2O
-
 ?
NH3 + a reduced acceptor + O2
-
 Acinetobacter sp. Y16 NH2OH + an acceptor + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
AMO
-
 Acinetobacter sp.
amoA
-
 Acinetobacter sp.
low-temperature ammonia monooxygenase
-
 Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Acinetobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 20 over 91% activity with in the range of 4-15°C, 15.1% of maximal activity at 20C, complete inactivation above 20°C Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Acinetobacter sp.