BRENDA - Enzyme Database show
show all sequences of 1.14.99.26

Metabolism of 2-hydroxypyridine by Bacillus brevis (INA)

Sharma, M.L.; Kaul, S.M.; Shukla, O.P; Biol. Mem. 9, 43-52 (1984)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
45 mM dithiothreitol affords partial protection against denaturation
Brevibacillus brevis
50% glycerol affords partial protection against denaturation
Brevibacillus brevis
dialysis of extracts in cold, 4C, against phosphate buffer, pH 7.0, 0.05 M, for 4 h, 90% activity loss
Brevibacillus brevis
enzyme is completely blocked by anaerobic conditions
Brevibacillus brevis
Inhibitors
Inhibitors
Commentary
Organism
Structure
FMN
85% inhibition at 1.7 mM, 2 min preincubation
Brevibacillus brevis
Na2As2O3
partial inhibition
Brevibacillus brevis
NaF
53% inhibition at 1.7 mM, 5 min preincubation
Brevibacillus brevis
NaN3
53% inhibition at 1.7 mM, 5 min preincubation
Brevibacillus brevis
NiCl2
47% inhibition at 1.7 mM
Brevibacillus brevis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Brevibacillus brevis
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-hydroxypyridine + NADH + O2
Brevibacillus brevis
-
2,5-dihydroxypyridine + NAD+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevibacillus brevis
-
isolated by enrichment on isonicotinic acid
-
Storage Stability
Storage Stability
Organism
-10C or 0C, overnight, complete loss of activity
Brevibacillus brevis
-20C, Tris HCl buffer, pH 7.4, 0.05 M, 10% ethanol, 50% glycerol, 0.045 M dithiothreitol, 1 mM 2-mercaptoethanol, stable for 16 d
Brevibacillus brevis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxypyridine + NADH + O2
-
438293
Brevibacillus brevis
2,5-dihydroxypyridine + NAD+ + H2O
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.2
-
Brevibacillus brevis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.5
half-maximal activity at pH 6.3 and pH 8.5
Brevibacillus brevis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Brevibacillus brevis
NADH
-
Brevibacillus brevis
NADPH
2% activity of NADH
Brevibacillus brevis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Brevibacillus brevis
NADH
-
Brevibacillus brevis
NADPH
2% activity of NADH
Brevibacillus brevis
General Stability (protein specific)
General Stability
Organism
45 mM dithiothreitol affords partial protection against denaturation
Brevibacillus brevis
50% glycerol affords partial protection against denaturation
Brevibacillus brevis
dialysis of extracts in cold, 4C, against phosphate buffer, pH 7.0, 0.05 M, for 4 h, 90% activity loss
Brevibacillus brevis
enzyme is completely blocked by anaerobic conditions
Brevibacillus brevis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
FMN
85% inhibition at 1.7 mM, 2 min preincubation
Brevibacillus brevis
Na2As2O3
partial inhibition
Brevibacillus brevis
NaF
53% inhibition at 1.7 mM, 5 min preincubation
Brevibacillus brevis
NaN3
53% inhibition at 1.7 mM, 5 min preincubation
Brevibacillus brevis
NiCl2
47% inhibition at 1.7 mM
Brevibacillus brevis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Brevibacillus brevis
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-hydroxypyridine + NADH + O2
Brevibacillus brevis
-
2,5-dihydroxypyridine + NAD+ + H2O
-
-
?
Storage Stability (protein specific)
Storage Stability
Organism
-10C or 0C, overnight, complete loss of activity
Brevibacillus brevis
-20C, Tris HCl buffer, pH 7.4, 0.05 M, 10% ethanol, 50% glycerol, 0.045 M dithiothreitol, 1 mM 2-mercaptoethanol, stable for 16 d
Brevibacillus brevis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxypyridine + NADH + O2
-
438293
Brevibacillus brevis
2,5-dihydroxypyridine + NAD+ + H2O
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.2
-
Brevibacillus brevis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.5
half-maximal activity at pH 6.3 and pH 8.5
Brevibacillus brevis
Other publictions for EC 1.14.99.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
438293
Sharma
-
Metabolism of 2-hydroxypyridin ...
Brevibacillus brevis
Biol. Mem.
9
43-52
1984
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