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Literature summary for 1.14.99.24 extracted from

  • Hu, Y.; Van Der Geize, R.; Besra, G.; Gurcha, S.; Liu, A.; Rohde, M.; Singh, M.; Coates, A.
    3-Ketosteroid 9alpha-hydroxylase is an essential factor in the pathogenesis of Mycobacterium tuberculosis (2010), Mol. Microbiol., 75, 107-121.
    View publication on PubMed
Search for more literature for 1.14.99.24

Cloned(Commentary)

Cloned (Comment) Organism
genes kshA or Rv3526, and kshB or Rv3571 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information gene disruption by a two-step mutagenesis strategy, the constructed DELTAkshA and DELTAkshB deletion mutants of strain H37Rv are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources, and are also unable to metabolize the steroid substrate 5alpha-androstane-3,17-dione in contrast to the wild-type enzyme, phenotypes, overview Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+ Mycobacterium tuberculosis
-
 9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
 ?
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+ Mycobacterium tuberculosis H37Rv
-
 9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 genes kshA or Rv3526, and kshB or Rv3571
-
Mycobacterium tuberculosis H37Rv
-
 genes kshA or Rv3526, and kshB or Rv3571
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
-
 Mycobacterium tuberculosis 9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
 ?
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
-
 Mycobacterium tuberculosis H37Rv 9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
 ?

Subunits

Subunits Comment Organism
More KSH is a two-component Rieske-type oxygenase comprised of an oxygenase component KshA and a reductase component KshB Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
3-ketosteroid 9alpha-hydroxylase
-
 Mycobacterium tuberculosis
KSH
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism 3-ketosteroid 9alpha-hydroxylase is a critical pathogenicity factor of strainH37Rv involved in steroid and cholesterol catabolism Mycobacterium tuberculosis
additional information DELTAkshA and DELTAkshB deletion mutants of strain H37Rv are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources, and are also unable to metabolize the steroid substrate 5alpha-androstane-3,17-dione in contrast to the wild-type enzyme. The deletion of either of these genes leads to rapid death of the microorganism in murine infection models and in macrophages, showing that kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis Mycobacterium tuberculosis
physiological function genes kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis Mycobacterium tuberculosis