Literature summary for 1.14.99.22 extracted from

Drummond, C.; Molina, M.; Taliansky, S.; Breidenbach, C.; Fioravanti, C.
Effects of quinizarin and five synthesized derivatives on fifth larval instar midgut ecdysone 20-monooxygenase activity of the tobacco hornworm Manduca sexta (2014), Int. J. Zool., 2014, 261512 .

No PubMed abstract available

Search for more literature for 1.14.99.22

Activating Compound

Activating Compound	Comment	Organism	Structure
9-methoxy-1,4-anthraquinone	about 125% activity at 0.001 mM	Manduca sexta

Inhibitors

Inhibitors	Comment	Organism	Structure
1,4-anthraquinone	about 20% residual activity at 10 mM	Manduca sexta
2-methoxy-1,4-anthraquinone	about 18% residual activity at 1 mM	Manduca sexta
9-hydroxy-1,4-anthraquinone	about 40% residual activity at 10 mM	Manduca sexta
9-methoxy-1,4-anthraquinone	about 45% residual activity at 10 mM	Manduca sexta
additional information	2-hydroxy-1,4-anthraquinone is essentially without effect on enzyme activity	Manduca sexta
quinizarin	about 60% residual activity at 10 mM	Manduca sexta

Organism

Organism	UniProt	Comment	Textmining
Manduca sexta	-	tobacco hornworm	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
larva	-	Manduca sexta	-
midgut	fifth larval instar midgut	Manduca sexta	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ecdysone + NADPH + O2	-	Manduca sexta	20-hydroxyecdysone + NADP+ + H+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
E20M	-	Manduca sexta
ecdysone 20-monooxygenase	-	Manduca sexta

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.04	-	at pH 7.5 and 35°C	Manduca sexta	2-methoxy-1,4-anthraquinone
0.3	-	at pH 7.5 and 35°C	Manduca sexta	1,4-anthraquinone
1	-	at pH 7.5 and 35°C	Manduca sexta	9-hydroxy-1,4-anthraquinone
2	-	at pH 7.5 and 35°C	Manduca sexta	9-methoxy-1,4-anthraquinone
60	-	at pH 7.5 and 35°C	Manduca sexta	quinizarin

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Release 2023.1 (January 2023)