BRENDA - Enzyme Database
show all sequences of 1.14.99.22

Ecdysterone biosynthesis: a microsomal cytochrome-P-450-linked ecdysone 20-monooxygenase from tissues of the African migratory locust

Feyereisen, R.; Durst, F.; Eur. J. Biochem. 88, 37-47 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
20-hydroxyecdysone
competitive inhibition
Locusta migratoria migratorioides
7,8-Benzoflavone
-
Locusta migratoria migratorioides
CO
partially reversible by irradiation with monochromatic light
Locusta migratoria migratorioides
cytochrome c
-
Locusta migratoria migratorioides
ecdysterone
competitive inhibition
Locusta migratoria migratorioides
Metyrapone
-
Locusta migratoria migratorioides
NADP+
competitive inhibition
Locusta migratoria migratorioides
NEM
-
Locusta migratoria migratorioides
nicotinamide
-
Locusta migratoria migratorioides
oxidized cytochrome c
reversed by KCN
Locusta migratoria migratorioides
PCMB
-
Locusta migratoria migratorioides
Phospholipase A
-
Locusta migratoria migratorioides
Phospholipase C
-
Locusta migratoria migratorioides
Piperonylbutoxide
-
Locusta migratoria migratorioides
SKF 525A
-
Locusta migratoria migratorioides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00027
-
ecdysone
-
Locusta migratoria migratorioides
0.0105
-
NADPH
-
Locusta migratoria migratorioides
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
bound to sedimentable membrane
Locusta migratoria migratorioides
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ecdysone + NADPH + O2
Locusta migratoria migratorioides
serves as precursor of ecdysterone, the active moulting hormone in larval insects
20-hydroxyecdysone + NADP+ + H2O
-
Locusta migratoria migratorioides
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Locusta migratoria migratorioides
-
L. migratoria migratorioides: african migration locust
-
Reaction
Reaction
Commentary
Organism
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O
NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein
Locusta migratoria migratorioides
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fat body
larval
Locusta migratoria migratorioides
-
malpighian tubule
-
Locusta migratoria migratorioides
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000017
-
fat body
Locusta migratoria migratorioides
0.00000041
-
mid- and hindgut
Locusta migratoria migratorioides
0.00000075
-
microsomes, fat body
Locusta migratoria migratorioides
0.00000115
-
malphigian tubules
Locusta migratoria migratorioides
0.000003
-
microsomes, malphigian tubules
Locusta migratoria migratorioides
Storage Stability
Storage Stability
Organism
-30°C, microsomal preparations with 30% v/v glycerol
Locusta migratoria migratorioides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-ecdysone + NADPH + O2
3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
beta-ecdysone, ecdysterone
285452
Locusta migratoria migratorioides
?
ecdysone + NADPH + O2
serves as precursor of ecdysterone, the active moulting hormone in larval insects
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
-
285452
Locusta migratoria migratorioides
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Locusta migratoria migratorioides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Locusta migratoria migratorioides
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
essential
Locusta migratoria migratorioides
NADH
increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor
Locusta migratoria migratorioides
NADPH
NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored; ultimate hydrogen donor, cannot be substituted by NADH or ascorbate
Locusta migratoria migratorioides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
essential
Locusta migratoria migratorioides
NADH
increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor
Locusta migratoria migratorioides
NADPH
NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored; ultimate hydrogen donor, cannot be substituted by NADH or ascorbate
Locusta migratoria migratorioides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
20-hydroxyecdysone
competitive inhibition
Locusta migratoria migratorioides
7,8-Benzoflavone
-
Locusta migratoria migratorioides
CO
partially reversible by irradiation with monochromatic light
Locusta migratoria migratorioides
cytochrome c
-
Locusta migratoria migratorioides
ecdysterone
competitive inhibition
Locusta migratoria migratorioides
Metyrapone
-
Locusta migratoria migratorioides
NADP+
competitive inhibition
Locusta migratoria migratorioides
NEM
-
Locusta migratoria migratorioides
nicotinamide
-
Locusta migratoria migratorioides
oxidized cytochrome c
reversed by KCN
Locusta migratoria migratorioides
PCMB
-
Locusta migratoria migratorioides
Phospholipase A
-
Locusta migratoria migratorioides
Phospholipase C
-
Locusta migratoria migratorioides
Piperonylbutoxide
-
Locusta migratoria migratorioides
SKF 525A
-
Locusta migratoria migratorioides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00027
-
ecdysone
-
Locusta migratoria migratorioides
0.0105
-
NADPH
-
Locusta migratoria migratorioides
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
bound to sedimentable membrane
Locusta migratoria migratorioides
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ecdysone + NADPH + O2
Locusta migratoria migratorioides
serves as precursor of ecdysterone, the active moulting hormone in larval insects
20-hydroxyecdysone + NADP+ + H2O
-
Locusta migratoria migratorioides
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fat body
larval
Locusta migratoria migratorioides
-
malpighian tubule
-
Locusta migratoria migratorioides
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000017
-
fat body
Locusta migratoria migratorioides
0.00000041
-
mid- and hindgut
Locusta migratoria migratorioides
0.00000075
-
microsomes, fat body
Locusta migratoria migratorioides
0.00000115
-
malphigian tubules
Locusta migratoria migratorioides
0.000003
-
microsomes, malphigian tubules
Locusta migratoria migratorioides
Storage Stability (protein specific)
Storage Stability
Organism
-30°C, microsomal preparations with 30% v/v glycerol
Locusta migratoria migratorioides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-ecdysone + NADPH + O2
3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
beta-ecdysone, ecdysterone
285452
Locusta migratoria migratorioides
?
ecdysone + NADPH + O2
serves as precursor of ecdysterone, the active moulting hormone in larval insects
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
-
285452
Locusta migratoria migratorioides
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Locusta migratoria migratorioides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
-
Locusta migratoria migratorioides
Other publictions for EC 1.14.99.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745079
Kong
The P450 enzyme Shade mediate ...
Leptinotarsa decemlineata
Insect Mol. Biol.
23
632-643
2014
-
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1
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1
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3
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4
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1
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1
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1
1
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1
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4
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1
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-
-
-
-
-
-
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1
1
-
-
-
745123
Drummond
-
Effects of quinizarin and fiv ...
Manduca sexta
Int. J. Zool.
2014
261512
2014
1
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-
6
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1
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2
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1
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6
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2
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1
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728083
Marchal
RNAi-mediated knockdown of Sha ...
Schistocerca gregaria
J. Insect Physiol.
58
890-896
2012
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-
1
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1
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1
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1
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1
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2
2
-
-
-
714771
Drummond
Cyclic 3',5' guanosine monopho ...
Manduca sexta
Comp. Biochem. Physiol. B
157
33-38
2010
2
-
-
-
-
-
4
-
-
-
-
-
-
2
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4
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1
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2
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4
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4
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1
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-
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715820
Suzuki
Physiological requirements for ...
Bombyx mori
J. Insect Physiol.
56
673-677
2010
1
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-
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2
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2
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1
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2
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-
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-
1
1
1
1
-
-
715821
Vandock
Midgut and fatbody mitochondri ...
Manduca sexta
J. Insect Physiol.
56
774-779
2010
-
-
-
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1
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2
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3
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2
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2
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1
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1
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1
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2
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2
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2
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2
2
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697454
Maeda
Molecular cloning of ecdysone ...
Bombyx mori
Comp. Biochem. Physiol. B
149
507-516
2008
-
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1
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1
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3
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1
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1
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2
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1
2
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1
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1
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1
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699193
Rauschenbach
Role of ecdysone 20-monooxygen ...
Drosophila melanogaster, Drosophila virilis
J. Comp. Physiol. B
178
27-32
2008
4
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2
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6
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1
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2
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2
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4
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4
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4
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2
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1
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2
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2
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2
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1
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1
-
-
684740
Mitchell
Effects of the compounds 2-met ...
Aedes aegypti, Drosophila melanogaster, Manduca sexta
Arch. Insect Biochem. Physiol.
66
45-52
2007
-
-
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12
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3
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3
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7
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5
3
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6
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3
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5
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5
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12
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3
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3
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5
3
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6
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3
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690037
Chentsova
-
Ecdysone 20-monooxygenase acti ...
Drosophila virilis
Russ. J. Genet.
43
829-830
2007
1
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1
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671944
Rewitz
The Halloween genes code for c ...
Manduca sexta
Biochem. Soc. Trans.
34
1256-1260
2006
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675980
Rewitz
Developmental expression of Ma ...
Manduca sexta
Mol. Cell. Endocrinol.
247
166-174
2006
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4
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657574
Alekseeva
-
Ecdysone 20-monooxygenase acti ...
Ajuga reptans
Appl. Biochem. Microbiol.
40
135-139
2004
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1
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660345
Petryk
Shade is the Drosophila P450 e ...
Drosophila melanogaster
Proc. Natl. Acad. Sci. USA
100
13773-13778
2003
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1
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1
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4
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4
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1
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4
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1
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285459
Winter
A microsomal ecdysone-binding ...
Locusta migratoria migratorioides
Biol. Chem.
382
1541-1549
2001
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1
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1
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1
1
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3
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1
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1
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1
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2
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1
2
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1
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1
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1
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2
1
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285461
Horike
Molecular cloning of NADPH-cyt ...
Bombyx mori
Eur. J. Biochem.
267
6914-6920
2000
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1
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2
1
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5
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1
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1
1
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3
1
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2
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1
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3
1
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285460
Horike
Ecdysone 20-monooxygenase in e ...
Bombyx mori
Arch. Insect Biochem. Physiol.
41
9-17
1999
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2
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2
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1
1
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285462
Mitchell
-
Ecdysone 20-monooxygenase acti ...
Manduca sexta
Arch. Insect Biochem. Physiol.
41
24-32
1999
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1
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285455
Grebenok
-
Ecdysone 20-monooxygenase , a ...
Spinacia oleracea
Phytochemistry
42
927-933
1996
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1
2
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1
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1
1
2
2
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285457
Weirich
-
Ecdysone 20-hydroxylation in M ...
Manduca sexta
Arch. Insect Biochem. Physiol.
31
305-312
1996
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2
2
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5
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5
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4
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285456
Shergill
-
EPR spectroscopic characteriza ...
Spodoptera littoralis
Biochem. J.
307
719-728
1995
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285463
Boecking
-
Metabolism of 3-dehydroecdyson ...
Faxonius limosus
Eur. J. Entomol.
92
63-74
1995
-
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6
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6
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1
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285458
Darvas
-
Ecdysone 20-monooxygenase syst ...
Parasarcophaga argyrostoma, Sarcophaga bullata
Comp. Biochem. Physiol. B
105
765-773
1993
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4
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4
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2
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2
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6
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6
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2
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10
2
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4
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2
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285451
Keogh
Effects of the adenylate cycla ...
Drosophila melanogaster, Drosophila melanogaster Canton S, Manduca sexta
Experientia
48
39-41
1991
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6
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3
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6
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2
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9
3
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4
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9
3
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6
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2
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285454
Weirich
-
Ecdysone 20-monooxygenase ...
Manduca sexta
Methods Enzymol.
3
454-458
1985
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1
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4
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285453
Greenwood
Ecdysone 20-mono-oxygenase in ...
Schistocerca gregaria
Biochem. J.
223
837-847
1984
1
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8
1
1
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8
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1
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1
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285452
Feyereisen
Ecdysterone biosynthesis: a mi ...
Locusta migratoria migratorioides
Eur. J. Biochem.
88
37-47
1978
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15
2
1
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3
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1
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2
5
1
2
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1
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15
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1
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2
5
1
2
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1
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1
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285450
Johnson
The mechanism of C-20 hydroxyl ...
Schistocerca gregaria
Biochem. J.
168
513-520
1977
-
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3
1
1
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1
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