BRENDA - Enzyme Database show
show all sequences of 1.14.99.19

Enzymatic synthesis of plasmalogens. Characterization of the 1-O-alkyl-2-acyl-8n-glycero-3-phosphorylethanolamine desaturase from mucosa of hamster small intestine

Paltauf, F.; Holasek, A.; J. Biol. Chem. 248, 1609-1615 (1973)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
heat-lable factor of high molecular weight
contained in the 100000 * g supernatant
Mesocricetus auratus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Mesocricetus auratus
cholate
weak inhibition
Mesocricetus auratus
CN-
-
Mesocricetus auratus
EDTA
-
Mesocricetus auratus
menadione
-
Mesocricetus auratus
NaN3
-
Mesocricetus auratus
NEM
-
Mesocricetus auratus
oleate
weak inhibition
Mesocricetus auratus
p-chloromercuribenzoate
-
Mesocricetus auratus
taurocholate
-
Mesocricetus auratus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Mesocricetus auratus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Mesocricetus auratus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
Mesocricetus auratus
-
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
-
Mesocricetus auratus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mesocricetus auratus
-
adult animals of either sex
-
Reaction
Reaction
Commentary
Organism
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + reduced acceptor + O2 = O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + acceptor + 2 H2O
mixed function oxidase
Mesocricetus auratus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
small intestine mucosa
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000028
-
microsomes plus soluble fraction, enzyme requires a heat-labile factor of high molecular weight contained in the soluble fraction
Mesocricetus auratus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates: O-3-alkyl-2-acyl-sn-glycero-1-phosphoethanolamine, octadecylglycerol, octadecanal, octadecanol, stearic acid
438312
Mesocricetus auratus
?
-
-
-
-
additional information
electron donors: NADPH or NADH
438312
Mesocricetus auratus
?
-
-
-
-
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
-
438312
Mesocricetus auratus
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
-
438312
Mesocricetus auratus
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
enzyme assay
Mesocricetus auratus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
phosphate buffer
Mesocricetus auratus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Mesocricetus auratus
NADPH
or NADH
Mesocricetus auratus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
heat-lable factor of high molecular weight
contained in the 100000 * g supernatant
Mesocricetus auratus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Mesocricetus auratus
NADPH
or NADH
Mesocricetus auratus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Mesocricetus auratus
cholate
weak inhibition
Mesocricetus auratus
CN-
-
Mesocricetus auratus
EDTA
-
Mesocricetus auratus
menadione
-
Mesocricetus auratus
NaN3
-
Mesocricetus auratus
NEM
-
Mesocricetus auratus
oleate
weak inhibition
Mesocricetus auratus
p-chloromercuribenzoate
-
Mesocricetus auratus
taurocholate
-
Mesocricetus auratus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Mesocricetus auratus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Mesocricetus auratus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
Mesocricetus auratus
-
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
-
Mesocricetus auratus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
small intestine mucosa
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000028
-
microsomes plus soluble fraction, enzyme requires a heat-labile factor of high molecular weight contained in the soluble fraction
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates: O-3-alkyl-2-acyl-sn-glycero-1-phosphoethanolamine, octadecylglycerol, octadecanal, octadecanol, stearic acid
438312
Mesocricetus auratus
?
-
-
-
-
additional information
electron donors: NADPH or NADH
438312
Mesocricetus auratus
?
-
-
-
-
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
-
438312
Mesocricetus auratus
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
-
438312
Mesocricetus auratus
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
enzyme assay
Mesocricetus auratus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
phosphate buffer
Mesocricetus auratus
Other publictions for EC 1.14.99.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727110
Watschinger
Orphan enzymes in ether lipid ...
Homo sapiens
Biochimie
95
59-65
2013
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
438318
Das
A novel chemical synthesis of ...
Rattus norvegicus
J. Lipid Res.
37
2706-2714
1996
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
2
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
438319
Blank
Plasmanylethanolamine D1-desat ...
Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Methods Enzymol.
209
390-396
1992
4
-
-
-
-
1
4
-
4
-
-
-
-
4
-
-
-
-
-
6
-
-
8
-
4
-
-
-
-
-
-
8
-
-
-
4
-
-
8
-
-
1
-
4
-
-
4
-
-
-
-
-
-
-
-
6
-
-
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
438316
Weber
Formation of ether lipids and ...
Mus musculus, Rattus norvegicus
Biochim. Biophys. Acta
711
197-207
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
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-
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-
1
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438315
Wykle
Biosynthesis of plasmalogens b ...
Rattus norvegicus
Biochemistry
18
3512-3517
1979
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
1
-
-
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-
-
-
1
-
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-
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-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438317
Baumann
On the substrate specificity o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
66
717-724
1975
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
1
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-
2
-
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-
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-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438312
Paltauf
Enzymatic synthesis of plasmal ...
Mesocricetus auratus
J. Biol. Chem.
248
1609-1615
1973
1
-
-
-
-
-
10
-
1
1
-
1
-
1
-
-
-
1
-
1
1
-
3
-
1
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
10
-
-
1
1
-
1
-
-
-
-
-
1
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
438314
Lee
Dietary control of stearyl CoA ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
55
574-579
1973
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438313
Wykle
Evidence for a mixed function ...
Rattus norvegicus
J. Biol. Chem.
247
5442-5447
1972
2
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
3
-
-
-
2
-
-
3
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
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-
-