Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Putidamonooxin | - |
Pseudomonas putida |
General Stability | Organism |
---|---|
NADH, not NADPH or substrate stabilize the reductase | Pseudomonas putida |
substrate or substrate analogues stabilize putidamomooxin by preventing loss of Fe2+ | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Methoxybenzoate | - |
Pseudomonas putida | |
4-trifluoromethylbenzoate | - |
Pseudomonas putida | |
benzoate | competitive inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
4-Methoxybenzoate | - |
Pseudomonas putida | |
0.0019 | - |
O2 | + 4-methoxybenzoate | Pseudomonas putida | |
0.0038 | - |
O2 | + 3,4-dimethoxybenzoate | Pseudomonas putida | |
0.01 | - |
O2 | + 4-ethoxybenzoate | Pseudomonas putida | |
0.051 | - |
O2 | + N-methyl-4-aminobenzoate | Pseudomonas putida | |
0.055 | - |
O2 | + 4-methylbenzoate | Pseudomonas putida | |
0.077 | - |
N-methyl-4-aminobenzoate | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
4-hyroxybenzoate, reductase | Pseudomonas putida |
0.004 | - |
4-hydroxybenzoate, monooxygenase | Pseudomonas putida |
0.008 | - |
3-methoxybenzoate, 4-methoxybenzoate, cell-free extract | Pseudomonas putida |
0.009 | - |
N,N-dimethyl-4-aminobenzoate | Pseudomonas putida |
0.021 | - |
4-hydroxy-3-methoxybenzoate | Pseudomonas putida |
0.023 | - |
3-hydroxybenzoate, reconstituted enzyme | Pseudomonas putida |
0.024 | - |
4-ethoxybenzoate, N-methyl-4-aminobenzoate | Pseudomonas putida |
0.025 | - |
3,4-dimethoxybenzoate | Pseudomonas putida |
0.04 | - |
4-methoxybenzoate, cell-free extract | Pseudomonas putida |
0.055 | - |
4-hyxdroxybenzoate | Pseudomonas putida |
0.077 | - |
4-hydroxybenzoate, reconstituted enzyme | Pseudomonas putida |
0.11 | - |
3-methoxybenzoate | Pseudomonas putida |
0.178 | - |
N-methyl-4-aminobenzoate | Pseudomonas putida |
0.314 | - |
4-ethoxybenzoate, piperonylate | Pseudomonas putida |
0.345 | - |
3,4-dimethoxybenzoate | Pseudomonas putida |
0.36 | - |
4-methoxybenzoate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dimethoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxy-3-methoxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
3,4-methylenedioxybenzoate + NADH + O2 | piperonylate | Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | protocatechuate | ? | |
3-hydroxybenzoate + NADH + O2 | partial uncoupler | Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | in the uncoupled part of the reaction, 3-hydroxybenzoate is not hydroxylated and H2O2 is a product, too | ? | |
3-methoxybenzoate + NADH + O2 | partial uncoupler | Pseudomonas putida | 3-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
4-ethoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + acetaldehyde + NAD+ + H2O | - |
? | |
4-Hydroxy-3-methoxybenzoate + NADH + O2 | vanillate, partial uncoupler | Pseudomonas putida | ? | - |
? | |
4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
alkylbenzoates | - |
Pseudomonas putida | ? | - |
? | |
additional information | - |
Pseudomonas putida | additional information | uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction | ? | |
N,N'-dimethyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? | |
N-methyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas putida | |
NADH | - |
Pseudomonas putida |