Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Putidamonooxin | - |
Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-Pyridylazo)resorcinol | - |
Pseudomonas putida | |
4-tert-Butylbenzoate | competitive inhibitor of the O-demethylation of 3-nitro-4-methoxybenzoate, hinders O2-binding or O2-activation | Pseudomonas putida | |
8-hydroxyquinoline | - |
Pseudomonas putida | |
Amytal | - |
Pseudomonas putida | |
Atebrin | - |
Pseudomonas putida | |
Bathocuproinedisulfonate | - |
Pseudomonas putida | |
bathophenanthrolinedisulfate | preferentially inhibiting putidamonooxin | Pseudomonas putida | |
Cd2+ | - |
Pseudomonas putida | |
Co2+ | - |
Pseudomonas putida | |
Cu2+ | - |
Pseudomonas putida | |
Cumylhydroperoxide | - |
Pseudomonas putida | |
Hg2+ | - |
Pseudomonas putida | |
Iodosobenzene | - |
Pseudomonas putida | |
Ni2+ | - |
Pseudomonas putida | |
p-chloromercuribenzoate | - |
Pseudomonas putida | |
rotenone | - |
Pseudomonas putida | |
Zn2+ | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00007 | - |
4-Methoxybenzoate | - |
Pseudomonas putida | |
0.0003 | - |
3-Nitro-4-methoxybenzoate | - |
Pseudomonas putida | |
0.00063 | - |
NADH | reconstituted enzyme | Pseudomonas putida | |
0.0058 | - |
4-methylaminobenzoate | - |
Pseudomonas putida | |
0.009 | - |
4-Methylbenzoate | - |
Pseudomonas putida | |
0.024 | - |
4-trifluoromethylbenzoate | - |
Pseudomonas putida | |
0.029 | - |
4-hydroxybenzoate | - |
Pseudomonas putida | |
0.055 | - |
O2 | + benzoate | Pseudomonas putida | |
0.14 | - |
NADPH | reconstituted enzyme | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.018 | - |
superoxide anion instead of substrate, putidamonooxin | Pseudomonas putida |
0.192 | - |
3-nitro-4-methoxybenzoate, putidamonooxin | Pseudomonas putida |
0.27 | - |
4-methoxybenzoate, putidamonooxin | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitro-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
3-nitro-4-methoxybenzoate + NADPH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NADP+ + H2O + formaldehyde | - |
r | |
4-hydroxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | ring hydroxylation | ? | |
4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
4-methylbenzoate + NADH + O2 | - |
Pseudomonas putida | 4-carboxybenzylalcohol + NAD+ + H2O | - |
? | |
4-trifluoromethylbenzoate + NADH + O2 | - |
Pseudomonas putida | ? | - |
? | |
N-methyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas putida | |
NADH | requirement, can be replaced by NADPH with 40% efficiency | Pseudomonas putida | |
NADPH | can replace NADH with 40% efficiency | Pseudomonas putida |