Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Putidamonooxin | essential part of the enzyme system, O2-activating 2Fe-2S-protein, identified by EPR and Mössbauer spectroscopy | Pseudomonas putida |
General Stability | Organism |
---|---|
substrate or substrate analogues stabilize putidamomooxin by preventing loss of Fe2+ | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Oxidized putidamonooxin | 60% inhibition with 3-nitro-4-methoxybenzoate as substrate, fully reactivated by Fe2+ and sulfhydryl-reagents | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | mononuclear non-heme iron protein, 2Fe-2S cluster | Pseudomonas putida | |
Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Oxidation Stability | Organism |
---|---|
putidamonooxin and NADH-reductase, extremely O2-sensitive, GSH, DTT, 2-mercaptoethanol prevent putidamonooxin oxidation of putdamonooxin | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O | a terminal oxygenase | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-amino-3-hydroxybenzoate + NAD+ + H2O | - |
? | |
4-hydroxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
4-methylbenzoate + NADH + O2 | p-toluate, partial uncoupler | Pseudomonas putida | 4-carboxybenzylalcohol + NAD+ + H2O | in the uncoupled part of the reaction, p-toluate is not hydroxylated and H2O2 is a product, too | ? | |
additional information | overview: substrates that are not oxygenized while NADH-oxidation and O2-consumption are catalyzed, such as: benzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-hydroxybenzoate, 4-bromobenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-trifluoromethylbenzoate, 4-tert-butylbenzoate | Pseudomonas putida | additional information | uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | prosthetic group of the oxidoreductase | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |