BRENDA - Enzyme Database show
show all sequences of 1.14.99.14

Microbial hydroxylations. V. 11alpha-hydroxylation of progesterone by cell-free preparations of Aspergillus ochraceus

Shibahara, M.; Moody, J.A.; Smith, L.L.; Biochim. Biophys. Acta 202, 172-179 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
11alpha-hydroxyprogesterone
induced by
Aspergillus ochraceus
nortestosterone
induced by
Aspergillus ochraceus
progesterone
best inducer
Aspergillus ochraceus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
progesterone + NADPH + O2
Bacillus megaterium
-
11alpha-hydroxyprogesterone + NADP+ + H2O
-
Bacillus megaterium
?
progesterone + NADPH + O2
Aspergillus ochraceus
-
11alpha-hydroxyprogesterone + NADP+ + H2O
-
Aspergillus ochraceus
?
progesterone + NADPH + O2
Aspergillus ochraceus NRRL 405
-
11alpha-hydroxyprogesterone + NADP+ + H2O
-
Aspergillus ochraceus NRRL 405
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus ochraceus
-
-
-
Aspergillus ochraceus NRRL 405
-
-
-
Bacillus megaterium
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus ochraceus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11alpha-hydroxyprogesterone + NADPH + O2
-
438354
Aspergillus ochraceus
6beta,11alpha-dihydroxyprogesterone
-
438354
Aspergillus ochraceus
?
11alpha-hydroxyprogesterone + NADPH + O2
-
438354
Aspergillus ochraceus NRRL 405
6beta,11alpha-dihydroxyprogesterone
-
438354
Aspergillus ochraceus NRRL 405
?
19-nortestosterone + NADPH + O2
-
438354
Aspergillus ochraceus
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
-
-
-
?
19-nortestosterone + NADPH + O2
-
438354
Aspergillus ochraceus NRRL 405
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
-
-
-
?
additional information
11alpha-hydroxylase induced by progesterone or 19-nortestosterone is free from associated 6beta-hydroxylase or 17beta-hydroxysteroid dehydrogenase activity, but induction with 11alpha-hydroxyprogesterone yields an enzyme with both 6beta- and 11alpha-hydroxylase activity
438354
Aspergillus ochraceus
?
-
-
-
-
additional information
11alpha-hydroxylase induced by progesterone or 19-nortestosterone is free from associated 6beta-hydroxylase or 17beta-hydroxysteroid dehydrogenase activity, but induction with 11alpha-hydroxyprogesterone yields an enzyme with both 6beta- and 11alpha-hydroxylase activity
438354
Aspergillus ochraceus NRRL 405
?
-
-
-
-
progesterone + NADPH + O2
-
438354
Bacillus megaterium
11alpha-hydroxyprogesterone + NADP+ + H2O
-
438354
Bacillus megaterium
?
progesterone + NADPH + O2
-
438354
Aspergillus ochraceus
11alpha-hydroxyprogesterone + NADP+ + H2O
-
438354
Aspergillus ochraceus
?
progesterone + NADPH + O2
-
438354
Aspergillus ochraceus NRRL 405
11alpha-hydroxyprogesterone + NADP+ + H2O
-
438354
Aspergillus ochraceus NRRL 405
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
loses potency at higher temperatures
Aspergillus ochraceus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
11alpha-hydroxyprogesterone
induced by
Aspergillus ochraceus
nortestosterone
induced by
Aspergillus ochraceus
progesterone
best inducer
Aspergillus ochraceus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
progesterone + NADPH + O2
Bacillus megaterium
-
11alpha-hydroxyprogesterone + NADP+ + H2O
-
Bacillus megaterium
?
progesterone + NADPH + O2
Aspergillus ochraceus
-
11alpha-hydroxyprogesterone + NADP+ + H2O
-
Aspergillus ochraceus
?
progesterone + NADPH + O2
Aspergillus ochraceus NRRL 405
-
11alpha-hydroxyprogesterone + NADP+ + H2O
-
Aspergillus ochraceus NRRL 405
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus ochraceus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11alpha-hydroxyprogesterone + NADPH + O2
-
438354
Aspergillus ochraceus
6beta,11alpha-dihydroxyprogesterone
-
438354
Aspergillus ochraceus
?
11alpha-hydroxyprogesterone + NADPH + O2
-
438354
Aspergillus ochraceus NRRL 405
6beta,11alpha-dihydroxyprogesterone
-
438354
Aspergillus ochraceus NRRL 405
?
19-nortestosterone + NADPH + O2
-
438354
Aspergillus ochraceus
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
-
-
-
?
19-nortestosterone + NADPH + O2
-
438354
Aspergillus ochraceus NRRL 405
11alpha-hydroxy-19-nortestosterone + NADP+ + H2O
-
-
-
?
additional information
11alpha-hydroxylase induced by progesterone or 19-nortestosterone is free from associated 6beta-hydroxylase or 17beta-hydroxysteroid dehydrogenase activity, but induction with 11alpha-hydroxyprogesterone yields an enzyme with both 6beta- and 11alpha-hydroxylase activity
438354
Aspergillus ochraceus
?
-
-
-
-
additional information
11alpha-hydroxylase induced by progesterone or 19-nortestosterone is free from associated 6beta-hydroxylase or 17beta-hydroxysteroid dehydrogenase activity, but induction with 11alpha-hydroxyprogesterone yields an enzyme with both 6beta- and 11alpha-hydroxylase activity
438354
Aspergillus ochraceus NRRL 405
?
-
-
-
-
progesterone + NADPH + O2
-
438354
Bacillus megaterium
11alpha-hydroxyprogesterone + NADP+ + H2O
-
438354
Bacillus megaterium
?
progesterone + NADPH + O2
-
438354
Aspergillus ochraceus
11alpha-hydroxyprogesterone + NADP+ + H2O
-
438354
Aspergillus ochraceus
?
progesterone + NADPH + O2
-
438354
Aspergillus ochraceus NRRL 405
11alpha-hydroxyprogesterone + NADP+ + H2O
-
438354
Aspergillus ochraceus NRRL 405
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
loses potency at higher temperatures
Aspergillus ochraceus
Other publictions for EC 1.14.99.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745647
Wang
Cloning and identification of ...
Absidia caerulea, Absidia caerulea AS3.65
J. Steroid Biochem. Mol. Biol.
171
254-261
2017
-
-
1
-
-
-
1
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746508
Gao
Screening for strains with 11 ...
Aspergillus ochraceus, Aspergillus ochraceus CICC41473, Beauveria bassiana, Beauveria bassiana ACCC30728, Cunninghamella elegans, Cunninghamella elegans CICC40250, Metarhizium anisopliae, Metarhizium anisopliae ACCC30194, Rhizopus stolonifer, Rhizopus stolonifer CGMCC33900
Steroids
124
67-71
2017
-
-
-
-
-
-
-
-
-
-
-
10
-
10
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744807
Chen
Molecular cloning, expression ...
Rhizopus oryzae, Rhizopus oryzae CICC 40467
Enzyme Microb. Technol.
66
28-34
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727305
Nguyen
Changing the regioselectivity ...
Bacillus megaterium
ChemBioChem
13
1161-1166
2012
-
1
-
-
15
-
-
14
1
-
1
2
-
1
-
-
-
-
-
-
-
-
3
1
1
-
-
15
-
-
-
-
-
-
-
-
1
-
-
-
15
-
-
-
-
14
1
-
1
2
-
-
-
-
-
-
-
-
3
1
1
-
-
15
-
-
-
-
-
-
-
-
15
15
715693
Petric
Discovery of a steroid 11alpha ...
Rhizopus oryzae, Rhizopus oryzae RA 99-880
J. Biotechnol.
150
428-437
2010
-
-
1
-
1
-
-
-
-
-
-
8
-
3
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
438363
Kunic
Comparison of two monooxygenas ...
Rhizopus stolonifer
Pflugers Arch.
439
R107-108
2000
-
-
-
-
-
-
2
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438362
Dutta
Bioconversion of progesterone ...
Aspergillus ochraceus, Aspergillus ochraceus G8, Aspergillus ochraceus TS, Rhizopus stolonifer
Curr. Microbiol.
39
309-312
1999
9
-
-
-
-
1
-
-
-
-
-
4
-
7
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438361
Lenasi
Identification and partial cha ...
Rhizopus stolonifer
Arch. Biochem. Biophys.
330
80-86
1996
-
-
-
-
-
1
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
438360
Bonnerjea
Poly(ethylene glycol) precipit ...
Rhizopus stolonifer
Appl. Microbiol. Biotechnol.
27
362-365
1988
-
1
-
-
-
-
-
-
3
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438359
Broad
Studies for the large scale is ...
Rhizopus stolonifer
Enzyme Microb. Technol.
9
546-548
1986
-
1
-
-
-
-
-
-
2
-
-
1
-
1
-
-
1
-
-
1
-
-
1
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-
-
-
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-
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1
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1
-
1
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-
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-
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2
-
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1
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1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438358
Cresnar
Resolution and reconstitution ...
Rhizopus stolonifer
Biochem. Biophys. Res. Commun.
133
1057-1063
1985
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
1
-
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1
1
-
-
-
-
-
-
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1
-
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-
1
-
-
-
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-
-
-
2
-
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1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288562
Jayanthi
Microsomal 11alpha-hydroxylati ...
Aspergillus ochraceus, Aspergillus ochraceus NRRL 405, Aspergillus ochraceus TS, Rhizopus oryzae, Rhizopus stolonifer
Biochem. Biophys. Res. Commun.
106
1262-1268
1982
-
-
-
-
-
-
7
-
4
-
-
5
-
9
-
-
-
-
-
2
2
-
8
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
5
-
-
-
-
7
-
-
4
-
-
5
-
-
-
-
-
2
2
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
438356
Ghosh
11alpha-Hydroxylation of proge ...
Aspergillus ochraceus, Aspergillus ochraceus TS
J. Steroid Biochem.
14
1063-1067
1981
2
-
-
-
-
-
1
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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-
-
1
-
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2
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1
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1
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2
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
438355
Hanisch
-
Regeneration of the progestero ...
Rhizopus stolonifer
Biotechnol. Lett.
2
123-126
1980
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
438354
Shibahara
Microbial hydroxylations. V. 1 ...
Aspergillus ochraceus, Aspergillus ochraceus NRRL 405, Bacillus megaterium
Biochim. Biophys. Acta
202
172-179
1979
3
-
-
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-
-
-
-
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3
-
4
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1
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9
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1
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3
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3
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1
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9
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1
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