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Literature summary for 1.14.99.1 extracted from
- Divergent cyclooxygenase responses to fatty acid structure and peroxide level in fish and mammalian prostaglandin H synthases (2006), FASEB J., 20, 1097-1108.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Ovis aries |
- |
Homo sapiens |
| expression in Spodoptera frugiperda Sf-9 cells | Salvelinus fontinalis |
| into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells | Salvelinus fontinalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Homo sapiens |  |
| 0.0012 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Homo sapiens | |
| 0.0017 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Homo sapiens | |
| 0.002 | - |
cis-8,11,14-eicosatrienoic acid | - |
Homo sapiens | |
| 0.0024 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Salvelinus fontinalis | |
| 0.0027 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Homo sapiens | |
| 0.003 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Ovis aries | |
| 0.0031 | - |
alpha-linolenic acid | - |
Homo sapiens | |
| 0.0031 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Ovis aries | |
| 0.0033 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Ovis aries | |
| 0.0048 | - |
alpha-linolenic acid | - |
Ovis aries | |
| 0.0048 | - |
gamma-linolenic acid | - |
Homo sapiens | |
| 0.0052 | - |
cis-11,14-eicosadienoic acid | - |
Homo sapiens | |
| 0.0055 | - |
cis-8,11,14-eicosatrienoic acid | - |
Ovis aries | |
| 0.0059 | - |
cis-8,11,14-eicosatrienoic acid | - |
Salvelinus fontinalis | |
| 0.007 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Ovis aries | |
| 0.0071 | - |
gamma-linolenic acid | - |
Ovis aries | |
| 0.0091 | - |
cis-11,14-eicosadienoic acid | - |
Ovis aries | |
| 0.0101 | - |
alpha-linolenic acid | - |
Salvelinus fontinalis | |
| 0.013 | - |
cis-5,8,11,14-eicosatetraenoic acid | - |
Salvelinus fontinalis | |
| 0.0153 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Salvelinus fontinalis | |
| 0.0318 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Salvelinus fontinalis | |
| 0.0357 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Salvelinus fontinalis | |
| 0.036 | - |
cis-8,11,14-eicosatrienoic acid | - |
Salvelinus fontinalis | |
| 0.039 | - |
cis-5,8,11,14,17-eicosapentaenoic acid | - |
Salvelinus fontinalis | |
| 0.061 | - |
cis-7,10,13,16-docosatetraenoic acid | - |
Salvelinus fontinalis | |
| 0.07 | - |
cis-4,7,10,13,16,19-docosahexaenoic acid | - |
Salvelinus fontinalis | |
| 0.082 | - |
alpha-linolenic acid | - |
Salvelinus fontinalis | |
| 0.162 | - |
gamma-linolenic acid | - |
Salvelinus fontinalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Salvelinus fontinalis | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + AH2 + O2 | Ovis aries | - |
prostaglandin H2 + A + H2O | - |
? | |
| arachidonate + AH2 + O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? | |
| arachidonate + AH2 + O2 | Salvelinus fontinalis | - |
prostaglandin H2 + A + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P23219 | PGHS-1 | - |
| Homo sapiens | P35354 | PGHS-2 | - |
| Ovis aries | - |
- |
- |
| Salvelinus fontinalis | Q9PTN3 | PGHS-1 | - |
| Salvelinus fontinalis | Q9PW89 | PGHS-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
| recombinant protein expressed in Sf9 cells | Homo sapiens |
| recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column | Salvelinus fontinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seminal vesicle | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2300 | - |
cyclooxygenase activity for partially purified prostaglandin H synthase 1 of Salvelinus fontinalis, tPGHS-1 | Salvelinus fontinalis |
| 15000 | - |
cyclooxygenase activity for partially purified prostaglandin H synthase 2 of Salvelinus fontinalis, tPGHS-2 | Salvelinus fontinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2 | - |
Ovis aries | ? + A + H2O | - |
? | |
| (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2 | - |
Salvelinus fontinalis | ? + A + H2O | - |
? | |
| alpha-linolenic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| alpha-linolenic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| arachidonate + AH2 + O2 | - |
Ovis aries | prostaglandin H2 + A + H2O | - |
? | |
| arachidonate + AH2 + O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? | |
| arachidonate + AH2 + O2 | - |
Salvelinus fontinalis | prostaglandin H2 + A + H2O | - |
? | |
| cis-11,14-eicosadienoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| cis-11,14-eicosadienoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| cis-4,7,10,13,16,19-docosahexaenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| cis-5,8,11,14,17-eicosapentaenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| cis-5,8,11,14,17-eicosapentaenoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| cis-5,8,11,14,17-eicosapentaenoic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| cis-5,8,11,14-eicosatetraenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| cis-7,10,13,16-docosatetraenoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| cis-7,10,13,16-docosatetraenoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| cis-7,10,13,16-docosatetraenoic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| cis-8,11,14-eicosatrienoic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| cis-8,11,14-eicosatrienoic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| cis-8,11,14-eicosatrienoic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| gamma-linolenic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
| gamma-linolenic acid + reduced acceptor + O2 | - |
Ovis aries | ? | - |
r | |
| gamma-linolenic acid + reduced acceptor + O2 | - |
Salvelinus fontinalis | ? | - |
r | |
| linolenic acid + AH2 + O2 | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hPGHS-1 | - |
Homo sapiens |
| hPGHS-2 | - |
Homo sapiens |
| oPGHS-1 | - |
Ovis aries |
| PGHS | - |
Ovis aries |
| PGHS | - |
Homo sapiens |
| PGHS | - |
Salvelinus fontinalis |
| PGHS-1 | - |
Ovis aries |
| PGHS-1 | - |
Homo sapiens |
| PGHS-1 | - |
Salvelinus fontinalis |
| PGHS-2 | - |
Homo sapiens |
| PGHS-2 | - |
Salvelinus fontinalis |
| prostaglandin H synthase | - |
Ovis aries |
| prostaglandin H synthase | - |
Homo sapiens |
| prostaglandin H synthase | - |
Salvelinus fontinalis |
| tPGHS-1 | brook trout, Salvelinus fontinalis | Salvelinus fontinalis |
| tPGHS-2 | brook trout, Salvelinus fontinalis | Salvelinus fontinalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
room temperature, 22-23°C, lipoxygenase activity assay | Homo sapiens |
| 23 | - |
room temperature, 22-23°C, lipoxygenase activity assay | Salvelinus fontinalis |
| 25 | - |
cyclooxygenase activity assay using a standard electrode | Ovis aries |
| 25 | - |
cyclooxygenase activity assay using a standard electrode | Homo sapiens |
| 25 | - |
cyclooxygenase activity assay using a standard electrode | Salvelinus fontinalis |
| 30 | - |
cyclooxygenase activity assay using a high sensitivity electrode | Ovis aries |
| 30 | - |
cyclooxygenase activity assay using a high sensitivity electrode | Homo sapiens |
| 30 | - |
cyclooxygenase activity assay using a high sensitivity electrode | Salvelinus fontinalis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 30 | - |
Ovis aries |
| 5 | 30 | - |
Homo sapiens |
| 5 | 30 | - |
Salvelinus fontinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
cyclooxygenase activity assay | Ovis aries |
| 7.2 | - |
cyclooxygenase activity assay | Homo sapiens |
| 7.2 | - |
cyclooxygenase activity assay | Salvelinus fontinalis |
| 8 | - |
lipoxygenase activity assay | Homo sapiens |
| 8 | - |
lipoxygenase activity assay | Salvelinus fontinalis |
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