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Literature summary for 1.14.20.9 extracted from

  • Zhu, J.; Lippa, G.; Gulick, A.; Tipton, P.
    Examining reaction specificity in PvcB, a source of diversity in isonitrile-containing natural products (2015), Biochemistry, 54, 2659-2669 .
    View publication on PubMed
Search for more literature for 1.14.20.9

Activating Compound

Activating Compound Comment Organism Structure
ascorbate
-
 Pseudomonas aeruginosa
ascorbate
-
 Erwinia amylovora

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.0 A resolution. The aromatic ring of the substrate binds in a hydrophobic pocket formed by Met114, Tyr115, and Leu116, with potential contributions from Trp83, as well. The carboxyl group of the tyrosine isonitrile does not interact directly with any positively charged residues Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 (2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate pH 7.8, 25°C Erwinia amylovora
0.023
-
 2-oxoglutarate pH 7.8, 25°C Pseudomonas aeruginosa
0.048
-
 2-oxoglutarate pH 7.8, 25°C Erwinia amylovora
0.093
-
 (2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate pH 7.8, 25°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron required Pseudomonas aeruginosa
Iron required Erwinia amylovora

Organism

Organism UniProt Comment Textmining
Erwinia amylovora D4I2N1
-
-
Erwinia amylovora CFBP1430 D4I2N1
-
-
Pseudomonas aeruginosa A0A0M3KL23
-
-
Pseudomonas aeruginosa PAK A0A0M3KL23
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + 2-oxoglutarate + O2
-
 Pseudomonas aeruginosa (2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate + succinate + CO2 + H2O
-
 ?
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + 2-oxoglutarate + O2
-
 Erwinia amylovora (2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate + succinate + CO2 + H2O
-
 ?
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + 2-oxoglutarate + O2
-
 Erwinia amylovora CFBP1430 (2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate + succinate + CO2 + H2O
-
 ?
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + 2-oxoglutarate + O2
-
 Pseudomonas aeruginosa PAK (2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate + succinate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
PAK_03057
-
 Pseudomonas aeruginosa
pvcB
-
 Pseudomonas aeruginosa
pvcB
-
 Erwinia amylovora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 2-oxoglutarate pH 7.8, 25°C Erwinia amylovora
2.3
-
 2-oxoglutarate pH 7.8, 25°C Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function enzyme is involved in biosynthesis of paerucumarin Pseudomonas aeruginosa
physiological function enzyme is involved in biosynthesis of paerucumarin Erwinia amylovora