Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutant enzymes | Pseudomonas savastanoi pv. phaseolicola |
Crystallization (Comment) | Organism |
---|---|
analysis of crystal structure of the enzyme in complex with several ligands, detailed overview | Pseudomonas savastanoi pv. phaseolicola |
Protein Variants | Comment | Organism |
---|---|---|
A198V | the mutant shows about 60% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
A198V | site-directed mutagenesis, the mutant produces large amounts of L-DELTA1-pyrroline-5-carboxylate but very little ethylene | Pseudomonas savastanoi pv. phaseolicola |
A218R | inactive | Pseudomonas savastanoi pv. phaseolicola |
A218V | the mutant shows about 3% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
A281V | site-directed mutagenesis, the mutant produces low levels of products in comparison to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
E213A | the mutant shows about 90% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
E213A/E215A | the mutant shows about 5% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
E215A | the mutant shows about 5% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
E285A | the mutant shows about 10% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
E285Q | the mutant shows about 25% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
F283A | the mutant shows about 20% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
F283A | site-directed mutagenesis, replacing F283 by tryptophan, tyrosine, arginine, alanine, and valine leads to the near elimination of ethylene production | Pseudomonas savastanoi pv. phaseolicola |
F283R | the mutant shows about 20% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
F283R | site-directed mutagenesis, replacing F283 by tryptophan, tyrosine, arginine, alanine, and valine leads to the near elimination of ethylene production | Pseudomonas savastanoi pv. phaseolicola |
F283V | the mutant shows about 20% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
F283V | site-directed mutagenesis, replacing F283 by tryptophan, tyrosine, arginine, alanine, and valine leads to the near elimination of ethylene production | Pseudomonas savastanoi pv. phaseolicola |
F283W | the mutant shows about 20% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
F283Y | inactive | Pseudomonas savastanoi pv. phaseolicola |
F283Y | site-directed mutagenesis, replacing F283 by tryptophan, tyrosine, arginine, alanine, and valine leads to the near elimination of ethylene production | Pseudomonas savastanoi pv. phaseolicola |
F310R | the mutant shows about 3% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
F310W | the mutant shows about 30% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
H116Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
H169Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
H233Q | site-directed mutagenesis, inactive mutant | Pseudomonas savastanoi pv. phaseolicola |
H284Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
H309Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
L73K | the mutant shows about 60% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
L73R | the mutant shows about 40% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
R171A | site-directed mutagenesis, the mutant is soluble, it produces no detectable ethylene | Pseudomonas savastanoi pv. phaseolicola |
R184A | the mutant shows about 75% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
R277A | site-directed mutagenesis, the mutant is expressed in inclusion bodies | Pseudomonas savastanoi pv. phaseolicola |
S81R | the mutant shows about 5% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
S81Y | the mutant shows about 5% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
V196F | inactive | Pseudomonas savastanoi pv. phaseolicola |
V196F | site-directed mutagenesis, the mutant is expressed in inclusion bodies | Pseudomonas savastanoi pv. phaseolicola |
V196R | inactive | Pseudomonas savastanoi pv. phaseolicola |
Y306A | the mutant shows about 3% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
Y306F | the mutant shows about 5% of wild type activity | Pseudomonas savastanoi pv. phaseolicola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-oxalylglycine | - |
Pseudomonas savastanoi pv. phaseolicola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Pseudomonas savastanoi pv. phaseolicola | |
Fe2+ | non-heme Fe(II)-dependent ethylene-forming enzyme, the metal ion is hexa-coordinated | Pseudomonas savastanoi pv. phaseolicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-arginine + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
2-oxoglutarate + L-arginine + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
succinate + CO2 + 5-hydroxy-L-arginine | - |
? | |
2-oxoglutarate + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
ethylene + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas savastanoi pv. phaseolicola | P32021 | - |
- |
Pseudomonas savastanoi pv. phaseolicola PK2 | P32021 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine + 2-oxoglutarate + O2 = succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | two-pathway reaction mechanism of EFE, structure-function relationship | Pseudomonas savastanoi pv. phaseolicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-arginine + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
2-oxoglutarate + L-arginine + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | succinate + CO2 + 5-hydroxy-L-arginine | - |
? | |
2-oxoglutarate + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | ethylene + CO2 | - |
? | |
3 2-oxoglutarate + L-arginine + 3 O2 | cf. EC 1.14.11.34, reaction, mechanism of the two reaction catalyzed at the same time, overview. Enzyme EFE converts 2-oxoglutarate into ethylene plus three CO2 molecules while also catalyzing the C5 hydroxylation of L-arginine driven by the oxidative decarboxylation of 2-oxoglutarate to form succinate and CO2 | Pseudomonas savastanoi pv. phaseolicola | 2 C2H4 + succinate + 7 CO2 + 3 H2O + guanidine + L-DELTA1-pyrroline-5-carboxylate | - |
? | |
additional information | substrate binding structures, crystal structure analysis, overview. In all cases of bound 2-oxoglutarate, the carboxylate distal to the metal is stabilized by a salt bridge with R277, and the carboxylate coordinating the metal is stabilized by hydrogen bonds with R171. The C1-carboxylate oxygen of 2-oxoglutarate binds approximately trans to the distal H268 and the C2-oxo oxygen binds opposite D191. L-Arg binds near, but does not coordinate, the metal center in EFE-Mn-2OG-L-Arg | Pseudomonas savastanoi pv. phaseolicola | ? | - |
? | |
additional information | substrate binding structures, crystal structure analysis, overview. In all cases of bound 2-oxoglutarate, the carboxylate distal to the metal is stabilized by a salt bridge with R277, and the carboxylate coordinating the metal is stabilized by hydrogen bonds with R171. The C1-carboxylate oxygen of 2-oxoglutarate binds approximately trans to the distal H268 and the C2-oxo oxygen binds opposite D191. L-Arg binds near, but does not coordinate, the metal center in EFE-Mn-2OG-L-Arg | Pseudomonas savastanoi pv. phaseolicola PK2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EFE | - |
Pseudomonas savastanoi pv. phaseolicola |
ethylene-forming enzyme | - |
Pseudomonas savastanoi pv. phaseolicola |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme EFE is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily. It contains a double-stranded beta-helix (DSBH, also known as the jellyroll or cupin fold) core typically found in members of the Fe(II)/2OG-dependent oxygenases | Pseudomonas savastanoi pv. phaseolicola |
physiological function | a non-heme Fe(II)- and 2-oxoglutarate-dependent ethylene-forming enzyme, EFE converts 2-oxoglutarate into ethylene plus three CO2 molecules while also catalyzing the C5 hydroxylation of L-arginine driven by the oxidative decarboxylation of 2-oxoglutarate to form succinate and CO2 | Pseudomonas savastanoi pv. phaseolicola |