Cloned (Comment) | Organism |
---|---|
gene efe, codon optimization, gene synthesis, and construction of the self-replicating wide-host-range pDF-series vectors, recombinant expression in Synechocystis sp. strain PCC 6803, the cyanobacterial system is highly unstable resulting in rapid development of mutants that lost the capability to synthesize ethylene, N-terminally His6-tagged enzyme expression in Escherichia coli. Ethylene synthesis in Escherichia coli and Synechocystis using three different metal-inducible promoters from cyanobacteria, expression rates and genomic structure of the native metal-inducible promoter elements, method development, overview | Pseudomonas syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-arginine + O2 | Pseudomonas syringae | - |
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
additional information | Pseudomonas syringae | in a few plant pathogens ethylene is synthesized by an ethylene forming enzyme in a complex multi-step reaction utilizing 2-oxoglutarate, arginine and dioxygen as substrates, resulting in the accumulation of ethylene in the headspace of closed vessels | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | - |
pv. phaseolicola | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-arginine + O2 | - |
Pseudomonas syringae | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
additional information | in a few plant pathogens ethylene is synthesized by an ethylene forming enzyme in a complex multi-step reaction utilizing 2-oxoglutarate, arginine and dioxygen as substrates, resulting in the accumulation of ethylene in the headspace of closed vessels | Pseudomonas syringae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-oxoglutarate-dependent ethylene/succinate-forming enzyme | - |
Pseudomonas syringae |
EFE | - |
Pseudomonas syringae |
ethylene forming enzyme | - |
Pseudomonas syringae |