Cloned (Comment) | Organism |
---|---|
gene GbFLS, DNA and amino acid sequence determination and analysis, promoter region sequence analysis and putative cis-acting regulatory elements, overview, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Ginkgo biloba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Ginkgo biloba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | Ginkgo biloba | - |
kaempferol + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ginkgo biloba | D0UZK7 | gene GbFLS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography | Ginkgo biloba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | - |
Ginkgo biloba | kaempferol + succinate + CO2 + H2O | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
Ginkgo biloba | kaempferol + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FLS | - |
Ginkgo biloba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ginkgo biloba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Ginkgo biloba |
Organism | Comment | Expression |
---|---|---|
Ginkgo biloba | GbFLS expression is induced by abiotic stresses: UV-B irradiation, abscisic acid, cold, sucrose, salicylic acid, and ethephon | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate iron-dependent oxygenase family | Ginkgo biloba |