Literature summary for 1.14.20.6 extracted from

Ferreyra, M.L.; Rius, S.; Emiliani, J.; Pourcel, L.; Feller, A.; Morohashi, K.; Casati, P.; Grotewold, E.
Cloning and characterization of a UV-B-inducible maize flavonol synthase (2010), Plant J., 62, 77-91.

Search for more literature for 1.14.20.6

Cloned(Commentary)

Cloned (Comment)	Organism
gene ZmFLS1, ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators in vivo, recombinant expression as His-tagged protein in Escherichia coli	Zea mays

Protein Variants

Protein Variants	Comment	Organism
H132F	site-directed mutagenesis, the mutant shows similar kinetics with dihydrokaempferol compared to the wild-type enzyme	Zea mays
additional information	ZmFLS1 partially complements the flavonol deficiency of the Arabidopsis thaliana fls1 mutant, and restores anthocyanin accumulation to normal levels. ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes	Zea mays

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0584	-	dihydrokaempferol	recombinant FLS1, pH not specified in the publication, temperature not specified in the publication	Zea mays
0.1511	-	dihydroquercetin	recombinant FLS1, pH not specified in the publication, temperature not specified in the publication	Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Zea mays	FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged FLS1 from Escherichia coli to about 80% purity by nickel affinity chromatography	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydrokaempferol + 2-oxoglutarate + O2	-	Zea mays	kaempferol + succinate + CO2 + H2O	-	?
dihydroquercetin + 2-oxoglutarate + O2	-	Zea mays	quercetin + succinate + CO2 + H2O	-	?
additional information	FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin	Zea mays	?	-	?

Synonyms

Synonyms	Comment	Organism
flavonol synthase	-	Zea mays
FLS1	-	Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.9	-	dihydroquercetin	recombinant FLS1, pH not specified in the publication, temperature not specified in the publication	Zea mays
6.6	-	dihydrokaempferol	recombinant FLS1, pH not specified in the publication, temperature not specified in the publication	Zea mays

Expression

Organism	Comment	Expression
Zea mays	the enzyme is UV-B-light-inducible in maize seedlings	up

General Information

General Information	Comment	Organism
metabolism	ZmFLS1 is a key flavonoid biosynthetic enzyme and involved in control of flavonol accumulation in maize	Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
26	-	dihydroquercetin	recombinant FLS1, pH not specified in the publication, temperature not specified in the publication	Zea mays
113	-	dihydrokaempferol	recombinant FLS1, pH not specified in the publication, temperature not specified in the publication	Zea mays

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Release 2023.1 (January 2023)