Literature summary for 1.14.20.6 extracted from

Lin, G.Z.; Lian, Y.J.; Ryu, J.H.; Sung, M.K.; Park, J.S.; Park, H.J.; Park, B.K.; Shin, J.S.; Lee, M.S.; Cheon, C.I.
Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli (2007), Protein Expr. Purif., 55, 287-292.

Search for more literature for 1.14.20.6

Application

Application	Comment	Organism
synthesis	production of active flavonol synthase from Camellia sinensis in Escherichia coli, with high specific activity	Camellia sinensis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, His-tag	Camellia sinensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.139	-	dihydroquercetin	pH 7.5, 30°C	Camellia sinensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	presence of Fe2+ in purification buffer is required to yield active enzyme	Camellia sinensis

Organism

Organism	UniProt	Comment	Textmining
Camellia sinensis	A2TJG2	-	-

Purification (Commentary)

Purification (Comment)	Organism
from inclusion bodies of recombinant Escherichia coli, denaturation with 6 M urea. Addition of FeSO4 to purification buffers significantly improves the recovery of active enzyme	Camellia sinensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydroquercetin + 2-oxoglutarate + O2	-	Camellia sinensis	quercetin + succinate + CO2 + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.16	-	dihydroquercetin	pH 7.5, 30°C	Camellia sinensis

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Release 2023.1 (January 2023)