Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oxoglutarate | no flavone synthase activity without this compound | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
RT-PCR cloning of flavone synthase cDNA, subcloning in pGEX 5X-2 for expression in Escherichia coli | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
S-naringenin | 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0 | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
FeSO4 | 65% reduced activity without FeSO4 | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38700 | - |
SDS-PAGE, 1027 bp, Fe2+-binding site, oxoglutarate binding site | Oryza sativa |
64700 | - |
SDS-PAGE, flavone synthase + glutathione S-transferase (26000 Da) | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-naringenin + 2-oxoglutarate + O2 | Oryza sativa | purified enzyme, 3 h, 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0 | apigenin + succinate + CO2 + H2O | apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expression of flavone synthase in Escherichia coli, purification on glutathione S-transferase (GST) affinity column | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-naringenin + 2-oxoglutarate + O2 | purified enzyme, 3 h, 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0 | Oryza sativa | apigenin + succinate + CO2 + H2O | apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin | ? |
Synonyms | Comment | Organism |
---|---|---|
flavone synthase | - |
Oryza sativa |
FNS | - |
Oryza sativa |
OsFNS | - |
Oryza sativa |