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Literature summary for 1.14.20.5 extracted from

  • Jeon, Y.M.; Kim, B.G.; Ahn, J.H.
    Biological synthesis of 7-O-methyl Apigenin from naringenin using escherichia coli expressing two genes (2009), J. Microbiol. Biotechnol., 19, 491-494.
    View publication on PubMed

Application

Application Comment Organism
biotechnology product of the combined activity of flavone synthase and flavone 7-O-methyltransferase produces genkwanin (7-O-methyl apigenin) which exhibits antibacterial activity against Vibrio cholerae and Enterococcus faecalis, and anti-inflammatory activity, direct plant extracts result in limited amounts Populus deltoides

Cloned(Commentary)

Cloned (Comment) Organism
the open reading frame of the flavone synthase gene PFNS-1 is cloned from Populus deltoids using RT-PCR, subcloned into Escherichia coli expression vector pGEX 5X-2, the flavone 7-O-methyltransferase into pRSF-Duet vector, both cotransformed into Escherichia coli BL21 Populus deltoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
SDS-PAGE, sum of flavone synthase and glutathione S-transferase (26000 Da) Populus deltoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
naringenin + O2 + 2-oxoglutarate Populus deltoides
-
apigenin + succinate + CO2 + H2O flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation ?

Organism

Organism UniProt Comment Textmining
Populus deltoides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
naringenin + O2 + 2-oxoglutarate
-
Populus deltoides apigenin + succinate + CO2 + H2O flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation ?

Synonyms

Synonyms Comment Organism
flavone synthase
-
Populus deltoides
PFNS-1
-
Populus deltoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
optimum for the combined action of both enzymes, next optimal pH is 8.0, then pH 7.0 Populus deltoides