Application | Comment | Organism |
---|---|---|
biotechnology | product of the combined activity of flavone synthase and flavone 7-O-methyltransferase produces genkwanin (7-O-methyl apigenin) which exhibits antibacterial activity against Vibrio cholerae and Enterococcus faecalis, and anti-inflammatory activity, direct plant extracts result in limited amounts | Populus deltoides |
Cloned (Comment) | Organism |
---|---|
the open reading frame of the flavone synthase gene PFNS-1 is cloned from Populus deltoids using RT-PCR, subcloned into Escherichia coli expression vector pGEX 5X-2, the flavone 7-O-methyltransferase into pRSF-Duet vector, both cotransformed into Escherichia coli BL21 | Populus deltoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
SDS-PAGE, sum of flavone synthase and glutathione S-transferase (26000 Da) | Populus deltoides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
naringenin + O2 + 2-oxoglutarate | Populus deltoides | - |
apigenin + succinate + CO2 + H2O | flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus deltoides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
naringenin + O2 + 2-oxoglutarate | - |
Populus deltoides | apigenin + succinate + CO2 + H2O | flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation | ? |
Synonyms | Comment | Organism |
---|---|---|
flavone synthase | - |
Populus deltoides |
PFNS-1 | - |
Populus deltoides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimum for the combined action of both enzymes, next optimal pH is 8.0, then pH 7.0 | Populus deltoides |