BRENDA - Enzyme Database
show all sequences of 1.14.20.4

Anthocyanidin synthase from Gerbera hybrida catalyzes the conversion of (+)-catechin to cyanidin and a novel procyanidin

Wellmann, F.; Griesser, M.; Schwab, W.; Martens, S.; Eisenreich, W.; Matern, U.; Lukacin, R.; FEBS Lett. 580, 1642-1648 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
maximal activity requires the addition of 0.3 mM ascorbate
Gerbera hybrid cultivar
Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Gerbera hybrid cultivar
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.175
-
(+)-catechin
pH 6.0, 37C
Gerbera hybrid cultivar
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
presence of ferrous iron is required
Gerbera hybrid cultivar
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gerbera hybrid cultivar
Q52MZ9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-catechin + 2-oxoglutarate + O2
-
744891
Gerbera hybrid cultivar
cyanidin + succinate + CO2 + 2 H2O
-
-
-
?
additional information
product formation is essentially dependent on the presence of ferrous ions and 2-oxoglutarate. No substrates: ent(-)-catechin (2S,3R-trans-catechin), (-)-epicatechin, ent(+)-epicatechin (2S,3S-cis-epicatechin) and (-)-gallocatechin
744891
Gerbera hybrid cultivar
?
-
-
-
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
maximal activity requires the addition of 0.3 mM ascorbate
Gerbera hybrid cultivar
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Gerbera hybrid cultivar
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.175
-
(+)-catechin
pH 6.0, 37C
Gerbera hybrid cultivar
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
presence of ferrous iron is required
Gerbera hybrid cultivar
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-catechin + 2-oxoglutarate + O2
-
744891
Gerbera hybrid cultivar
cyanidin + succinate + CO2 + 2 H2O
-
-
-
?
additional information
product formation is essentially dependent on the presence of ferrous ions and 2-oxoglutarate. No substrates: ent(-)-catechin (2S,3R-trans-catechin), (-)-epicatechin, ent(+)-epicatechin (2S,3S-cis-epicatechin) and (-)-gallocatechin
744891
Gerbera hybrid cultivar
?
-
-
-
-
Other publictions for EC 1.14.20.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745148
Giampieri
Overexpression of the anthocy ...
Matthiola incana
J. Agric. Food Chem.
66
581-592
2018
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1
1
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742637
Chawla
-
Anthocyanidin synthase in lea ...
Solanum melongena
Indian J. Agric. Biochem.
30
105-106
2017
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2
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742427
Kim
-
Identification of two novel m ...
Allium cepa
Euphytica
212
427-437
2016
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2
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1
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742599
Rafique
Nonsense mutation inside anth ...
Rubus idaeus
Front. Plant Sci.
7
1892
2016
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1
1
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743507
Zhang
The Reaumuria trigyna leucoan ...
Reaumuria trigyna
Plant Physiol. Biochem.
106
278-287
2016
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1
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4
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1
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1
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1
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1
1
1
1
-
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743789
Cao
-
Cloning and expression analys ...
Ficus carica
Sci. Hortic.
211
369-375
2016
-
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1
-
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-
-
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1
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7
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1
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7
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2
1
1
2
-
-
741548
Zhang
-
The expression level of antho ...
Malus hybrid cultivar
Acta Physiol. Plant.
37
109
2015
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2
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2
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1
2
4
2
-
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741645
Shi
Molecular characterization an ...
Magnolia sprengeri
Appl. Biochem. Biotechnol.
175
477-488
2015
-
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1
-
-
-
-
-
-
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7
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1
1
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742619
Li
Cloning of an anthocyanidin s ...
Ribes nigrum
Genet. Mol. Res.
14
2726-2734
2015
-
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1
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4
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2
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2
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1
1
1
1
-
-
743595
Ben-Simhon
A 'White' anthocyanin-less po ...
Punica granatum
PLoS ONE
10
e0142777
2015
-
-
-
-
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-
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5
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2
2
-
-
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744602
Liu
Proanthocyanidin synthesis in ...
Theobroma cacao
BMC Plant Biol.
13
202
2013
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1
1
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725953
Yan
-
Cloning and expression analysi ...
Brassica juncea
Mol. Breed.
28
313-322
2011
-
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1
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1
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2
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1
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1
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3
3
-
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-
713486
Wang
Expression and tissue and subc ...
Vitis vinifera, Vitis vinifera Cabernet Sauvignon
Protoplasma
248
267-279
2010
-
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3
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4
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6
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6
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1
1
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744453
Sahay
Structure prediction of dihyd ...
Spinacia oleracea
Bioinformation
5
259-263
2010
-
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1
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3
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697947
Preuss
Arabidopsis thaliana expresses ...
Arabidopsis thaliana
FEBS Lett.
583
1981-1986
2009
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1
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3
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1
1
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700225
Khlestkina
Anthocyanin biosynthesis genes ...
Secale cereale
Mol. Genet. Genomics
282
475-485
2009
-
1
1
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4
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700767
Zhou
-
Molecular cloning and expressi ...
Ipomoea batatas
Plant Mol. Biol. Rep.
28
112-121
2009
-
1
1
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1
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1
1
1
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1
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1
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3
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700864
Stracke
Metabolomic and genetic analys ...
Arabidopsis thaliana
Planta
229
427-445
2009
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700867
Szankowski
Shift in polyphenol profile an ...
Malus domestica
Planta
229
681-692
2009
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700212
Xu
Molecular cloning and function ...
Ginkgo biloba
Mol. Cells
26
536-547
2008
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1
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2
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701213
Nakatsuka
-
Isolation and expression analy ...
Rhododendron x pulchrum
Sci. Hortic.
118
314-320
2008
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1
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1
1
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1
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688073
Zhou
Ultraviolet A-specific inducti ...
Brassica rapa
J. Exp. Bot.
58
1771-1781
2007
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2
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688823
Reddy
Novel transgenic rice overexpr ...
Oryza sativa
Metab. Eng.
9
95-111
2007
-
-
1
-
1
-
-
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-
1
1
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5
-
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1
1
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1
1
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689527
Cheng
-
Characterization of anthocyani ...
Saussurea medusa
Plant Cell Tissue Organ Cult.
89
63-73
2007
-
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1
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1
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1
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3
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689595
Pang
Early steps in proanthocyanidi ...
Medicago truncatula
Plant Physiol.
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