Literature summary for 1.14.20.3 extracted from

Clifton, I.J.; Doan, L.X.; Sleeman, M.C.; Topf, M.; Suzuki, H.; Wilmouth, R.C.; Schofield, C.J.
Crystal structure of carbapenem synthase (CarC) (2003), J. Biol. Chem., 278, 20843-20850.

Search for more literature for 1.14.20.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Pectobacterium carotovorum

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor diffusion method. Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221), consistent with solution studies. CarC monomers contain a double-stranded beta-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure is obtained with L-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems are used to investigate substrate binding	Pectobacterium carotovorum

Crystallization (Comment)

Organism

hanging-drop vapor diffusion method. Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221), consistent with solution studies. CarC monomers contain a double-stranded beta-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure is obtained with L-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems are used to investigate substrate binding

Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
200000	-	gel filtration	Pectobacterium carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum	Q9XB59	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pectobacterium carotovorum

Subunits

Subunits	Comment	Organism
hexamer	CarC crystallizes as a hexamer comprised of two trimers. Predominant form of CarC in solution is also hexameric with low levels of monomeric and trimeric forms also being observed	Pectobacterium carotovorum

Synonyms

Synonyms	Comment	Organism
CarC	-	Pectobacterium carotovorum

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Release 2023.1 (January 2023)