Literature summary for 1.14.20.15 extracted from

Blasiak, L.C.; Vaillancourt, F.H.; Walsh, C.T.; Drennan, C.L.
Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis (2006), Nature, 440, 368-371 .

Search for more literature for 1.14.20.15

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas syringae pv. syringae

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of SyrB2 with both a chloride ion and 2-oxoglutarate coordinated to the iron ion at 1.6 A resolution	Pseudomonas syringae pv. syringae

Protein Variants

Protein Variants	Comment	Organism
A118D	mutant enzyme binds Fe(II), but the mutations completely abrogates chlorination activity	Pseudomonas syringae pv. syringae
A118E	mutant enzyme binds Fe(II), but the mutations completely abrogates chlorination activity	Pseudomonas syringae pv. syringae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	non-haem Fe(II)-dependent enzyme, mononuclear iron enzyme. Crystallographic data confirm that halide ions bind directly to iron, before decarboxylation of 2-oxoglutarate and formation of a reactive ferryl-oxo species	Pseudomonas syringae pv. syringae

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas syringae pv. syringae	Q9RBY6	-	-
Pseudomonas syringae pv. syringae B301D	Q9RBY6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas syringae pv. syringae

Synonyms

Synonyms	Comment	Organism
SyrB2	-	Pseudomonas syringae pv. syringae

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in syringomycin E biosynthesis	Pseudomonas syringae pv. syringae

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Release 2023.1 (January 2023)